Description: UltraComp eBeads® react with antibodies of mouse, rat and hamster origin, and are immunoglobulin light chain-independent. They are designed for use in compensation with all fluorochromes excited by ultraviolet (355 nm) violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633-640 nm) lasers. The beads are spherical particles that can be stained with individual fluorochrome-conjugated antibodies for use as single-color compensation controls.
Each drop of beads contains two populations: a positive population that will capture any mouse, rat or hamster antibody and a negative population that will not react with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations result. This bimodal distribution can be used for single-color compensation controls in multicolor flow cytometry experiments.
UltraComp eBeads are compatible with all fluorochromes excited by an ultraviolet (355 nm) or violet (405 nm) laser. They should be used with standard staining buffers which contain PBS or HBSS, protein such as BSA or FBS, and sodium azide. Do not use UltraComp eBeads with Super Bright Staining Buffer or other additives. For more information, please contact technical support.