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InstantOne™ ELISA

The 1-hour, 1-wash phospho ELISA

InstantOne, the 1-hour, 1-wash cell signaling assay, detects total and phosphorylated targets with an easy-to-use assay that results in sensitivity that exceeds industry standards.

In this assay, the target analyte binds to the two sandwich ELISA antibodies in solution, as the capture antibody binds to the plate through a proprietary mechanism, so both the sample and the assay reagents may be added to the InstantOne ELISA assay plate at the same time. Unbound assay reagents and nonspecific sample components are washed away (just as in a traditional sandwich ELISA), while the specific analyte is detected through a colorimetric detection.

Learn more about the InstantOne ELISA Technology and Workflow

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Name Cat. No. Reg.  

AKT Pathway Activation Profile InstantOne™ ELISA

85-86048 RUO

phospho-AKT 1/2/3 (Ser473) InstantOne™ ELISA

85-86042 RUO

AKT 1 (Total) InstantOne™ ELISA

85-86047 RUO

AKT (Total/Phospho) InstantOne™ ELISA

85-86046 RUO

phospho-AKT 1/2/3 (Thr308) InstantOne™ ELISA

85-86044 RUO

AKT/ERK Activation InstantOne™ ELISA

85-86014 RUO

ERK/AKT/p70 S6K Activation InstantOne™ ELISA

85-86018 RUO

beta-Catenin (Total/Phospho) InstantOne™ ELISA

85-86143 RUO

beta-Catenin (Total) InstantOne™ ELISA

85-86141 RUO

phospho-beta-Catenin (Ser45) InstantOne™ ELISA

85-86142 RUO

CREB (Total/Phospho) InstantOne™ ELISA

85-86153 RUO

CREB (Total) InstantOne™ ELISA

85-86151 RUO

phospho-CREB (Ser133) InstantOne™ ELISA

85-86152 RUO

ERK 1/2 (Total/Phospho) InstantOne™ ELISA

85-86013 RUO

ERK 1/2 (Total) InstantOne™ ELISA

85-86011 RUO

phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) InstantOne™ ELISA

85-86012 RUO


85-86131 RUO

phospho-GSK3 beta (Ser9) InstantOne™ ELISA

85-86172 RUO

GSK3 beta (Total) InstantOne™ ELISA

85-86171 RUO

GSK3 beta (Total/Phospho) InstantOne™ ELISA

85-86173 RUO

IkBa (Total/Phospho) InstantOne™ ELISA

85-86063 RUO

IkBa (Total) InstantOne™ ELISA

85-86061 RUO

phospho-IkBa (Ser32/36) InstantOne™ ELISA

85-86062 RUO

phospho-IKKa (Ser176/180) InstantOne™ ELISA (Discontinued)

85-86072 RUO

IKKa (Total) InstantOne™ ELISA (Discontinued)

85-86071 RUO

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* = Please click on product link to see all available sizes and pricing.

FC = Flow Cytometry, Intracellular Staining/Flow Cytometry; ELISA = ELISA, ELISPOT; Multiplex = Multiplex Immunoassays; ICC = Immunocytochemistry; IHC = Immunohistochemistry, Immunofluorescence, Microscopy, Imaging, In Vivo Imaging; IHC-F = Immunohistochemical Staining of Frozen Tissue Sections; IHC-P = Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections; FA = Functional Assays, Bioassays, Neutralization, Depletion Studies, Biomolecule Conjugation; IP = Immunoprecipitation; WB = Western Blotting

RUO = Research Use Only; GPR = General Purpose Reagent; ASR = Analyte Specific Reagent. Analytical and performance characteristics are not established; CE = CE-marked reagents