The gold standard clone for detection of human KLRG1
NK and CD8 T cells have been shown to share many of the same inhibitory receptors which have become extremely useful in both the functional and phenotypic characterization of these cells types. Among these is the inhibitory molecule, killer cell lectin-like receptor G1 (KLRG1). In humans, KLRG1 has been shown to be expressed at high levels by both NK and some CD8 T cells, as well as mast cells and basophils. KLRG1 has been used as a key marker of CD8 T cell senescence and terminal effector differentiation in mice; however, less is known about the function of KLRG1 in human cells. Similar to mouse, studies have shown KLRG1+ CD8 T cells to be more senescent with less proliferative potential than their KLRG1– counterparts.
To date, there have been no commercially available options for the investigation of human KLRG1 expression by flow cytometry, making easy and robust detection of this molecule difficult. Now, the well-established standards for the detection of KLRG1 by flow cytometry are both available from eBioscience: Clones 13F12F2 and 13A2 developed by Dr. Hanspeter Pircher at the University of Freiburg (Voehringer, 2002).
Staining of normal human peripheral blood cells with Anti-Human CD8a APC (cat. 17-0088) and Mouse IgG2a K Isotype Control PE (cat. 12-4724) (left) or Anti-Human KLRG1 PE (right). Cells in the lymphocyte gate were used for analysis.