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Immunoprecipitation and Western Blot

Immunoprecipitation (IP)

Immunoprecipitation (IP) is an enrichment procedure of specific proteins, protein modifications, or protein motifs from a heterogeneous protein sample such as cell lysate. The enrichment occurs through the use of an antibody that commonly recognizes a protein in its native or semi-native format. IP is often followed by a western blot for analysis, but can be followed by other applications as well. IP allows for the detection of proteins of low abundance, protein:protein interactions and protein post-translational modifications (PTMs).

Western Blot (WB)

Western Blot (WB), also referred to as immunoblot, is a common, effective method for determining relative protein or protein-modification levels in a sample. Denatured proteins are separated via SDS-PAGE and transferred to a membrane where the proteins are detected via the use of antibodies. The relative intensity, and location of the protein on the blot, allow for its identification and quantification.


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