ViewRNA miRNA ISH Cell Assay- How It Works
mRNA and miRNA in situ hybridization assay workflow
The ViewRNA miRNA ISH Cell Assay is based on patent-pending probe design and bDNA signal amplification technology.
The ViewRNA assay can be performed on cover slips processed in a 24-well plate or in a 96-well plate. The assay has four main steps: sample preparation, target hybridization with probes, signal amplification using bDNA technology, and detection. At the end of the assay the processed cover slips are mounted on standard microscope slides for imaging or if the 96-well plate was used, no further processing for imaging is required.
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Specificity of the miRNA probe in the miRNA in situ hybridization assay
The ability of the ViewRNA miRNA ISH Cell Assay to distinguish highly homologous targets with a 3-base difference was evaluated in situ with synthetic miRNAs transfected in HeLa cells. Very low levels of non-specific signal were observed for many miRNAs differing by three bases (Figure 1, Table I). Read More …
Specificity of the mRNA probe in the mRNA in situ hybridization assay
A highly specific and sensitive signal can be achieved by using target-specific probe sets comprised of 20 oligonucleotide pairs. Read More ...