PrimeFlow® RNA Assay
Enter a new dimension of single-cell analysis
PrimeFlow® RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cell changes over time or in response to stimulus. This novel assay employs a proprietary fluorescent in situ hybridization (FISH) technique enabling simultaneous detection of up to three RNA transcripts in a single cell using a standard flow cytometer. RNA detection may be teamed together with intracellular and cell surface antibody staining to elevate the understanding of single cell dynamics to a new dimension.
Development of the assay is based upon proven and well-published Affymetrix® ViewRNA™ FISH assays designed for microscopic analysis of RNA in cells and tissues. The assay combines paired oligonucleotide probe design with branched DNA (bDNA) signal amplification to robustly detect gene expression at the single-cell level.
Coupling RNA expression with protein detection on a flow cytometer generates multiparametric data in heterogeneous cell populations and offers in-depth and high content details at the single cell level. In contrast, microarrays and sequencing can provide comprehensive gene expression data in bulk sample preparations; however, such bulk sample preps can mask the individual effects of different cellular subsets. Using the PrimeFlow® RNA Assay, specific cell populations may be analyzed for unique transcript expression levels or cell subsets evaluated over time to determine transcriptional regulation and protein expression simultaneously. Such unique and valuable insights are highly applicable to answering previously unanswerable questions and have broad implications for advancing research across multiple fields of biology.
- Observe gene expression heterogeneity at the single cell level
- Compare kinetics of both RNA and protein expression in the same cell
- Detect non-coding RNA in cellular subsets
- Evaluate viral RNA in infected cells
- Analyze mRNA expression levels when antibody selection is limited
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Designed for specific and sensitive detection of RNA by in situ hybridization.