Anti-Vimentin eFluor® 615

Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments. Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, as well as pancreatic precursor cells. It is proposed to be a marker of cardiac differentiation. In neural cells, vimentin expression is gradually replaced by neurofilaments. Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils.

This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin.

References: Moisan E, Girard D. Cell surface expression of intermediate filament proteins vimentin and lamin B1 in human neutrophil spontaneous apoptosis. J Leukoc Biol. 2006 Mar;79(3):489-98. (V9, FC)

Dubey M, Hoda S, Chan WK, Pimenta A, Ortiz DD, Shea TB. Reexpression of vimentin in differentiated neuroblastoma cells enhances elongation of axonal neurites. Neurosci Res. 2004 Oct 15;78(2):245-9. (V9, ICC)

Bohn W, Wiegers W, Beuttenmüller M, Traub P. Species-specific recognition patterns of monoclonal antibodies directed against vimentin. Exp Cell Res. 1992 Jul;201(1):1-7. (V9, IHC)