anti-ssDNA (APOSTAIN)

Description: Monoclonal antibody F7-26 used in this procedure was generated against calf thymus ssDNA and selected on the basis of reactivity with mouse apoptotic cells. This antibody specifically reacts with deoxycytidine and requires for its binding ssDNA of at least 25-30 bases in length. MAb F7-26 is not species-specific and could be used to detect apoptotic cells in various species (mouse, rat and human cells were tested).

Choose APOSTAIN Anti-ssDNA to specifically stain condensed chromatin of apoptotic cells, a hallmark of apoptosis. Unlike other apoptosis assays and kits, this unique reagent provides specifi city based on the high sensitivity of DNA in the condensed chromatin of apoptotic cells to thermal denaturation. Staining of cells and/or tissue sections with APOSTAIN following heat treatment, induces DNA denaturation in situ only in apoptotic nuclei. In the presence of formamide, only apoptotic (and not necrotic) nuclei DNA becomes denatured and detectable. Thus, APOSTAIN is a specifi c and sensitive method for the detection of apoptotic cells.

Citations: Verzola,D.; Gandolfo,M.; Ferrario,F.; Rastaldi,M.; Villaggio,B.; Gianiorio,F.; Giannoni,M.; Rimoldi,L.; Lauria,F.; Miji,M.; Deferrari,G.; Garibotto,G.. Apoptosis in the kidneys of patients with type II diabetic nephropathy. Kidney Int 2007;72:1262-1272. (Link)

Amrein,Lilian; Loignon,Martin; Goulet,Anne Christine; Dunn,Michael; Jean-Claude,Bertrand; Aloyz,Raquel; Panasci,Lawrence. Chlorambucil Cytotoxicity in Malignant B Lymphocytes Is Synergistically Increased by 2-(Morpholin-4-yl)-benzo[h]chomen-4-one (NU7026)-Mediated Inhibition of DNA Double-Strand Break Repair via Inhibition of DNA-Dependent Protein Kinase. Journal of Pharmacology and Experimental Therapeutics 2007;321:848-855. (Link)

Villanacci,V.; Bassotti,G.; Cathomas,G.; Maurer,C.A.; Di Fabio,F.; Fisogni,S.; Cadei,M.; Mazzocchi,A.; Salerni,B.. Is pseudomelanosis coli a marker of colonic neuropathy in severely constipated patients?. Histopathology 2006;49:132-137. (Link)

Tuoheti,Y.; Itoi,E.; Pradhan,R.L.; Wakabayashi,I.; Takahashi,S.; Minagawa,H.; Kobayashi,M.; Okada,K.; Shimada,Y.. Apoptosis in the supraspinatus tendon with stage II subacromial impingement. Journal of Shoulder and Elbow Surgery 2005;14:535-541. (Link)

Ralton,L.D.; Bestwick,C.S.; Milne,L.; Duthie,S.; Kong Thoo Lin,P.. Bisnaphthalimidopropyl spermidine induces apoptosis within colon carcinoma cells. Chemico-Biological Interactions 2009;177:1-6. (Link)

Bassotti,Gabrio; Villanacci,Vincenzo; Fisogni,Simona; Cadei,Morris; Di Fabio,Francesco; Salerni,Bruno. Apoptotic phenomena are not a major cause of enteric neuronal loss in constipated patients with dementia. Neuropathology 2007;27:67-72. (Link)