Mitochondrial Membrane Potential Dyes Chart
|JC-1||515 nm||530 nm (monomer/ depolarized)
590 nm (aggregates/ polarized)
|Measures polarization/ depolarization of mitochondrial membrane||
Selectively enters mitochondria & can be used to indicate the initiation of apoptosis.
Changes in fluorescence is reversible.
Compatible with flow cytometry and fluorescent spectrophotometry.
Provides qualitative and quantitative measurements.
|Compensation between the monomer and aggregate emission can be difficult.|