Mitochondrial Membrane Potential Dyes Chart
| Dye | Excitation | Emission | Function | Benefits | Limitations |
|---|---|---|---|---|---|
| JC-1 | 515 nm | 530 nm (monomer/ depolarized) 590 nm (aggregates/ polarized) |
Measures polarization/ depolarization of mitochondrial membrane |
Selectively enters mitochondria & can be used to indicate the initiation of apoptosis. Changes in fluorescence is reversible. Compatible with flow cytometry and fluorescent spectrophotometry. Provides qualitative and quantitative measurements. |
Compensation between the monomer and aggregate emission can be difficult. |