**Starting now: ebioscience.com products are on thermofisher.com. Ebioscience.com to retire in April. Get details >


DNA Content, Cell Cycle Analysis, Nucleated Cells Identification Dyes Chart

DNA Content, Cell Cycle Analysis, and Identification of Nucleated Cells Dyes
DyeExcitationEmissionFunctionBenefitsLimitations
DRAQ5™ 488-647 nm 670 nm Labels dsDNA

Binds DNA stoichiometrically for analysis of cell cycle and ploidy.

Does not require fixation/ permeabilization.

Can be used as a nuclear counterstain for microscopy.

Can be used to distinguish nucleated/nonnucleated cells for flow cytometry.

Broad excitation/
emission spectra may limit use in multi-color flow cytometry & may not be compatible with PerCP-eFluor® 710, PerCP-Cy5.5, PE-Cy7, APC, Alexa Fluor® 647, Alexa Fluor® 700, APC-eFluor 780, or APC-Alexa Fluor® 750.

For cell cycle analysis, emission should be collected with far-red filters (780/60) to obtain optimal CVs.

For cell cycle analysis, it may be necessary to label cells for a longer period of time and with a higher concentration to obtain optimal CVs.

CyTRAK Orange™ 488-550 nm 610 nm Labels dsDNA

Binds DNA.

Does not require fixation/permeabilization.

Can be used to distinguish nucleated and nonnucleated cells for flow cytometry.

Background staining of the cytoplasm may limit its use as a nuclear counterstain in fluorescent microscopy.
Propidium Iodide 488 nm 617 nm Labels dsDNA/RNA

Binds DNA/RNA stoichiometrically for analysis of cell cycle and ploidy.

May also be used for live/dead discrimination in flow cytometry.

For cell cycle analysis or DNA binding, must fix/permeabilize cells.

Requires degradation of cellular RNA for DNA/cell cycle analysis.

Has a broad emission spectra making it difficult to use in multi-color flow cytometric applications.

7-AAD 548 nm 647 nm Labels dsDNA

Specifically binds DNA (does not bind to RNA) stoichiometrically for analysis of cell cycle and ploidy.

May also be used for live/dead discrimination in flow cytometry.

For cell cycle analysis or DNA binding, must fix/permeabilize cells.