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Cell Tracking and Labeling Dyes Chart

Cell Tracking and Cell Labeling Dyes
DyeExcitationEmissionFunctionBenefitsLimitations
CFSE 494 nm 521 nm Labels cytoplasmic proteins

Good for short- & long-term labeling of cells (days to weeks) for flow cytometry or microscopy.

Can be used to track cell division history (see Cell Proliferation Dyes).

Labeling cells with too much dye may inhibit function & significantly increases compensation values (flow cytometry).

Cannot be used in combination with FITC, Alexa Fluor® 488, or GFP.

Cell Proliferation Dye eFluor® 450 409 nm 450 nm Labels cellular proteins

Can identify up to 7 individual cell divisions as dye is divided equally between daughter cells.

Compatible with GFP.

Labeling cells with too much dye may inhibit function & also significantly increases compensation values.

Cannot be used on combination with Pacific Blue or eFluor 450.

Cell Proliferation Dye eFluor® 670 647 nm 670 nm 
Labels cellular proteins

Good for short- & long-term labeling of cells (days to weeks) for flow cytometry or microscopy.

Can be used to track cell division history (see Cell Proliferation Dyes).

Compatible with GFP.

Labeling cells with too much dye may inhibit function & significantly increases compensation values (flow cytometry).

Cannot be used in combination with APC or Alexa Fluor® 647.

CellVue® Jade 478 nm 508 nm Labels cell membrane

Good for short term labeling of cells (1-7 days) for flow cytometry or microscopy.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.

Does not divide evenly between daughter cells for tracking cell division.

Cannot be used in combination with FITC, Alexa Fluor® 488, or GFP.

CellVue® Lavender 420 nm 461 nm Labels cell membrane

Good for short term labeling of cells (1-2 days) for flow cytometry or microscopy.

The dimmest of the CellVue dyes.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.

Does not divide evenly between daughter cells for tracking cell division.

Cannot be used in combination with eFluor® 450 or Pacific Blue®.

CellVue® Maroon 647 nm 667 nm Labels cell membrane

Good for short term labeling of cells (1-7 days) for flow cytometry or microscopy.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.

Does not divide evenly between daughter cells for tracking cell division.

May not be compatible with other 633 nm-excitable fluorochromes.

CellVue® Burgundy 683 nm 707 nm Labels cell membrane

Good for short term labeling of cells (1-7 days) for flow cytometry or microscopy.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.

Does not divide evenly between daughter cells for tracking cell division.

May not be compatible with other 633 nm-excitable fluorochromes.

CellVue® Plum 652 nm 671 nm Labels cell membrane

Good for short term labeling of cells (1-7 days) for flow cytometry or microscopy.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.

Does not divide evenly between daughter cells for tracking cell division.

May not be compatible with other 633 nm-excitable fluorochromes.

CellVue® NIR780 745 nm 776 nm Labels cell membrane

Good for short term labeling of cells (1-7 days) for flow cytometry (can be excited by the red laser line) or microscopy.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.

Does not divide evenly between daughter cells for tracking cell division.

Cannot be used in combination with APC-eFluor® 780 or APC-Alexa Fluor® 750.

CellVue® NIR815 786 nm 814 nm Labels cell membrane

Good for short term labeling of cells (1-7 days) for in vivo imaging.

Labeling cells with too much dye or for too long will kill cells.

Dye can be transferred to other cells through trogocytosis.