Cell Proliferation and Division Dyes Chart
| Dye | Excitation | Emission | Function | Benefits | Limitations |
|---|---|---|---|---|---|
| CFSE | 494 nm | 521 nm | Labels cytoplasmic proteins |
Can identify up to 8 individual cell divisions as dye is divided equally between daughter cells. |
Labeling cells with too much dye may inhibit function & significantly increases compensation values. Cannot be used in combination with FITC, Alexa Fluor® 488, or GFP. |
| Cell Proliferation Dye eFluor® 450 | 409 nm | 450 nm | Labels cellular proteins |
Can identify up to 7 individual cell divisions as dye is divided equally between daughter cells. Compatible with GFP. |
Labeling cells with too much dye may inhibit function & also significantly increases compensation values. Cannot be used in combination with Pacific Blue® or eFluor 450 |
| Cell Proliferation Dye eFluor® 670 |
647 nm | 660 nm | Labels cellular proteins |
Can identify up to 6 individual cell divisions as dye is divided equally between daughter cells. Compatible with GFP. |
Labeling cells with too much dye may inhibit function & also significantly increases compensation values. Cannot be used in combination with APC or Alexa Fluor® 647. |
