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Cell Proliferation and Division Dyes Chart

Cell Proliferation and Cell Division Dyes
DyeExcitationEmissionFunctionBenefitsLimitations
CFSE 494 nm 521 nm Labels cytoplasmic proteins

Can identify up to 8 individual cell divisions as dye is divided equally between daughter cells.

Labeling cells with too much dye may inhibit function & significantly increases compensation values.

Cannot be used in combination with FITC, Alexa Fluor® 488, or GFP.

Cell Proliferation Dye eFluor® 450 409 nm 450 nm Labels cellular proteins

Can identify up to 7 individual cell divisions as dye is divided equally between daughter cells.

Compatible with GFP.

Labeling cells with too much dye may inhibit function & also significantly increases compensation values.

Cannot be used in combination with Pacific Blue® or eFluor 450

Cell Proliferation Dye
eFluor® 670
647 nm 660 nm Labels cellular proteins

Can identify up to 6 individual cell divisions as dye is divided equally between daughter cells.

Compatible with GFP.

Labeling cells with too much dye may inhibit function & also significantly increases compensation values.

Cannot be used in combination with APC or Alexa Fluor® 647.