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General Frequently Asked Questions


Antibody Product Line

Q. What type of antibodies do you offer?

eBioscience offers a wide range of monoclonal and polyclonal antibodies for detecting murine, human, non-human primate and other species of antigens. Antibodies can be used for the detection of cell surface markers, such as CD molecules, cytoskeletal and cytoplasmic proteins, chemokine receptors, as well as intracellular cytokines/chemokines and intra-nuclear proteins including transcription factors.

Q. What is the shelf life of your antibodies?

The majority of eBioscience antibodies have a minimum guaranteed shelf life of at least 6 months from date of receipt. Please refer to vial label for expiration date. This guarantee is provided if the products are kept under optimal storage conditions as stated on the Technical Data Sheet. If you are not completely satisfied with the performance of your product or have a question about the expiration date, please contact technical support at tech@ebioscience.com for assistance.

Q. What information do you have regarding the cross-reactivity of your antibodies?

Information about known cross-reactivity of an antibody is given on the Technical Data Sheets. We have done some in-house testing to analyze cross-reactivity of anti-human antibodies amongst non-human primates. The results of these tests are summarized in our Cross-Reactivity Charts.

Q. What formats do you offer?

For convenience, eBioscience offers antibodies in a variety of formats, Best Protocols® Fluorescent Dyes Chart. We carry purified and biotin conjugates with and without sodium azide, and a large number of direct fluorochrome conjugates. Our newest additions are eFluor® Nanocrystals (NC) and eFluor® Organic Dyes, for more information please click here. These include eFluor 605NC, 625NC, 650NC (all nanocrystal reagents) as well as eFluor 450 (Pacific Blue® replacement), APC-eFluor 780 (APC-Alexa® Fluor 750 replacement), eFluor 660 (Alexa Fluor 647 replacement) and PerCP-eFluor 710 .

Q. What is the difference between your Affinity Purified and Functional Grade Purified formats?

The Affinity Purified format of our monoclonal antibodies contains no additional protein carriers such as BSA or gelatin but does contain sodium azide as a preservative. These products can be used for flow cytometry, WB, IP, and IHC applications. The Functional Grade purified formats contain no sodium azide and are tested for endotoxin. These products meet or exceed the industry standard of 0.001 ng endotoxin/ug antibody and in most instances have endotoxin levels significantly lower than these standards (unless otherwise stated on the product technical datasheet). Please contact tech@ebioscience.com if you would like lot specific information for Functional Grade products. The Functional Grade formats are generally used for bioassays and in vivo studies.

Q. What are the concentrations of your antibodies?

eBioscience offers both test and microgram to milligram sizes, depending on the product. The concentration of the test sizes is indicated on the Technical Data Sheets under Applications Tested. The concentrations of the microgram sizes vary depending on the format. Please refer to the product vial for lot specific information.

Q. What applications can I use eBioscience antibodies for?

eBioscience antibodies can be used for a variety of applications, such as:

  1. Flow cytometric analysis- surface antigen staining (FC)
  2. Flow cytometric analysis- intracellular staining (IC Flow)
  3. Immunohistochemistry (IHC)
  4. Immunohistochemistry- frozen sections (IH/F)
  5. Immunohistochemistry- paraffin sections (IHC-Paraffin)
  6. Immunoblotting (WB)
  7. Immunoprecipitation (IP)
  8. ELISA
  9. ELISPOT
  10. Bioassays (FA)
  11. Neutralization/Blocking studies (NU)
  12. In vitro Cytokine Capture Assay (IVCCA)
  13. In vivo studies in mice
  14. Chromatin immunoprecipitation (ChIP)

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