Multiplexing Protocols

Bead Coupling for FlowCytomix™ Multiplex Bead Based Assays

The protocol of coupling antibodies to polystyrene beads for FlowCytomix is proprietary know-how of eBioscience and cannot be disclosed. However, various bead coupling protocols are described in the literature. An example can be found here:

Siiman O et al. Covalently Bound Antibody on Polystyrene Latex Beads: Formation, Stability, and Use in Analyses of White Blood Cell Populations. Journal of Colloid and Interface Science 2001;234:44-58.