Bead Coupling for FlowCytomix™ Multiplex Bead Based Assays
The protocol of coupling antibodies to polystyrene beads for FlowCytomix is proprietary know-how of eBioscience and cannot be disclosed. However, various bead coupling protocols are described in the literature. An example can be found here:
Siiman O et al. Covalently Bound Antibody on Polystyrene Latex Beads: Formation, Stability, and Use in Analyses of White Blood Cell Populations. Journal of Colloid and Interface Science 2001;234:44-58.A general protocol is available in conjunction with raw beads upon request by contacting email@example.com.