Flow Cytometry (FACS) Protocols

Annexin V Staining Protocol

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  • Protocol A: Annexin V Staining
  • Protocol B: Annexin V Staining with Fixable Viability Dyes
  • Protocol C: Annexin V Staining with Surface and Intracellular Staining

BrdU Staining for Flow Cytometry

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Cell Preparation for Flow Cytometry Protocols

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  • Protocol A: Cell Preparation of Tissue Culture Cells
  • Protocol B: Cell Preparation from Lymphoid Tissue
  • Protocol C: Cell Preparation from Non-Lymphoid Tissue
  • Protocol D: Isolation of PBMC from Whole Blood

Compensation Beads for Flow Cytometry

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Pharmacological Induction of Apoptosis With Camptothecin Protocol

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Proliferation Dyes for Flow Cytometry

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  • Protocol A: Cell Proliferation Dye eFluor® 670
  • Protocol B: Cell Proliferation Dye eFluor® 450
  • Protocol C: CFSE

Red Blood Cell Lysis Protocol

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  • Protocol A: 1X or 10X RBC Lysis Buffer
  • Protocol B: 1-step Fix/Lyse Solution

Staining Cell Surface Antigens for Flow Cytometry Protocol

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  • Protocol A: Cell Suspensions
  • Protocol B: Human Lysed Whole Blood

Staining Intracellular Antigens for Flow Cytometry Protocol

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  • Protocol A: Two step protocol for (cytoplasmic) intracellular proteins
  • Protocol B: One step protocol for (nuclear) intracellular proteins
  • Protocol C: Two-step protocol: Fixation/Methanol
  • Click here to see the Intracellular Staining Guide Chart

Viability Staining Protocol

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  • Protocol A: Staining Dead Cells with Propidium Iodide or 7-AAD
  • Protocol B: Staining Live Cells with Calcein Dyes
  • Protocol C: Staining Dead Cells with eBioscience Fixable Viability eFluor® Dyes