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Flow Cytometry (FACS) Protocols

Flow Cytometry (FACS) Protocols

Annexin V Staining Protocol

Protocol A: Annexin V Staining
Protocol B: Annexin V Staining with Fixable Viability Dyes
Protocol C: Annexin V Staining with Surface and Intracellular Staining

BrdU Staining for Flow Cytometry

Cell Preparation for Flow Cytometry Protocols

Protocol A: Cell Preparation of Tissue Culture Cells
Protocol B: Cell Preparation from Lymphoid Tissue
Protocol C: Cell Preparation from Non-Lymphoid Tissue
Protocol D: Isolation of PBMC from Whole Blood

Pharmacological Induction of Apoptosis With Camptothecin Protocol

Red Blood Cell Lysis Protocol

Protocol A: RBC Lysis of Mouse Splenocytes
Protocol B: RBC Lysis of Mouse Blood
Protocol C: RBC Lysis of Human Peripheral Blood
Protocol D: Lysis of Human Blood for Flow Cytometric Analysis

Staining Cell Surface Antigens for Flow Cytometry Protocol

Protocol A: Cell Suspensions
Protocol B: Human Lysed Whole Blood

Staining Intracellular Antigens for Flow Cytometry Protocol

Protocol A: Two step protocol for (cytoplasmic) intracellular proteins
Protocol B: One step protocol for (nuclear) intracellular proteins
Click here to see the Intracellular Staining Guide Chart

Viability Staining Protocol

Protocol A: Staining Dead Cells with Propidium Iodide or 7-AAD
Protocol B: Staining Live Cells with Calcein Dyes
Protocol C: Staining Dead Cells with eBioscience Fixable Viability eFluor® Dyes

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