Rat TNF alpha ELISA Ready-SET-Go!®

Also known as: Tumor Necrosis Factor alpha, TNFa TNF-a, TNF-alpha

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7340

Cat. No.	Size
88-7340-22	2 x 96 tests w/plates
88-7340-76	20 x 96 tests w/plates
88-7340-77	20 x 96 tests
88-7340-86	10 x 96 tests w/plates
88-7340-88	10 x 96 tests

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Description

Description: The Rat TNF-α ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of rat TNF-α protein levels from samples including serum, plasma, and supernatants from cell cultures.

Details

Reactivity	Rat
Sensitivity	16 pg/ml
Standard Curve Range	16 pg/ml - 2000 pg/ml
Components	Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications	ELISA

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

References

References: Lehmann GL, Carreras FI, Soria LR, Gradilone SA, Marinelli RA. LPS induces the TNF-alpha-mediated downregulation of rat liver aquaporin-8: role in sepsis-associated cholestasis. Am J Physiol Gastrointest Liver Physiol. 2008 Feb;294(2):G567-75. (RSG ELISA, serum, PubMed)

Zhang W, Lu C, et al. 2007. Therapeutic effect of combined triptolide and glycyrrhizin treatment on rats with collagen induced arthritis. Planta Med. 73(4):336-40. (RSG ELISA, serum, PubMed)

Zeng Y, Gu B, et al. 2007. Sinomenine, an antirheumatic alkaloid, ameliorates clinical signs of disease in the Lewis rat model of acute experimental autoimmune encephalolmyelitis. Biol Pharm Bull. 30(8):1438-44. (RSG ELISA, TC supernatant, PubMed)

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Antigens	Area of Biology	Cell Type
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells