Description: This FlowCytomix Simplex Kit is designed for the measurement of MIP-1alpha in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Rat Basic Kit (cat. BMS8441FF), this kit can be used to detect MIP-1alpha alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
In this kit, the antibodies are mouse-specific and the standards are recombinant mouse proteins. The antibodies are cross-reactive with rat cytokines.
This kit contains bead population A11.
Chemokines are cytokines that induce chemotaxis of inflammatory cells. They are able to induce leukocyte chemotaxis and adhesion to endothelial cells.
MIP-1alpha and MIP-1beta belong to the family of cysteine-cysteine (cc) chemokines, RANTES being another prominent member thereof.
Both MIP-1alpha and MIP-1beta are not only chemoattractants but also coactivators of macrophages acting in concert with IFN-gamma as type 1 cytokines.
MIP-1alpha and MIP-1beta are distinct but highly homologous chemokines produced by a variety of host cells in response to various external stimuli and share affinity for their receptor CCR5. The roles of MIP-1alpha and MIP-1beta have been elucidated in response to their affects on cellular and humoral immune response. MIP-1alpha was shown to stimulate strong antigen specific responses, while MIP-1beta promotes antibody responses. Both macrophage inflammatory proteins are however strictly associated with type 1 immune response.
Determination of the expression levels of the MIP-1s turned out to provide important information regarding numerous diseases such as multiple myeloma, allergic asthmatic disorders, acute experimental autoimmune encephalomyelitis, HIV infection, sarcoidosis and sepsis.