Rat IL-10 Platinum ELISA

Also known as: TGIF, MCSF III, CSIF, B TCGF

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS629*

Cat. No. Size
BMS629 96 tests
BMS629TWO 2 x 96 tests
BMS629FIVE 5 x 96 tests
BMS629TEN 10 x 96 tests
Please inquire about custom configurations or bulk packaging.

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Data for Rat IL-10 Platinum ELISA.

Click on image to view more information.

  • Data for Rat IL-10 Platinum ELISA.
Description

Description: The rat IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-10. The rat IL-10 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.

Details
Reactivity Rat
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum
Sensitivity 1.5 pg/mL
Standard Curve Range 15.6 - 1,000 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-10
Biotin-Conjugate anti-rat IL-10 monoclonal antibody
Streptavidin-HRP
Rat IL-10 Standard lyophilized, 2 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Reported Applications ELISA
Documentation
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
BMS622* Rat TNF-alpha Platinum ELISA ELISA RUO
BMS339* Recombinant Rat IL-4 RUO
BMS86085FF* Mouse IgE FlowCytomix Simplex Kit 488 - 561 nm 575 nm Multiplex RUO
References

Citations: Yang,Ying; Mou,Yun; Hu,Shen Jiang; Fu,Michael. Beneficial effect of rosuvastatin on cardiac dysfunction is associated with alterations in calcium-regulatory proteins. European Journal of Heart Failure 2009;11:6-13. (Link)

References: Heinzel,F.P.; Sadick,M.D.; Mutha,S.S.; Locksley,R.M.. Production of interferon gamma, interleukin 2, interleukin 4, and interleukin 10 by CD4+ lymphocytes in vivo during healing and progressive murine leishmaniasis. Proc.Natl.Acad.Sci.U.S.A 1991;88:7011-7015. (Link)

Barnes,P.F.; Chatterjee,D.; Abrams,J.S.; Lu,S.; Wang,E.; Yamamura,M.; Brennan,P.J.; Modlin,R.L.. Cytokine production induced by Mycobacterium tuberculosis lipoarabinomannan. Relationship to chemical structure. J.Immunol. 1992;149:541-547. (Link)

Kim,J.M.; Brannan,C.I.; Copeland,N.G.; Jenkins,N.A.; Khan,T.A.; Moore,K.W.. Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes. J.Immunol. 1992;148:3618-3623. (Link)

Mosier,D.E.; Yetter,R.A.; Morse,H.C.,III. Retroviral induction of acute lymphoproliferative disease and profound immunosuppression in adult C57BL/6 mice. J.Exp.Med. 1985;161:766-784. (Link)

Basu,S.; Dunn,A.; Ward,A.. G-CSF: function and modes of action (Review). Int.J.Mol.Med. 2002;10:3-10. (Link)

Vieira,P.; de Waal-Malefyt,R.; Dang,M.N.; Johnson,K.E.; Kastelein,R.; Fiorentino,D.F.; deVries,J.E.; Roncarolo,M.G.; Mosmann,T.R.; Moore,K.W.. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. Proc.Natl.Acad.Sci.U.S.A 1991;88:1172-1176. (Link)

Moore,K.W.; Vieira,P.; Fiorentino,D.F.; Trounstine,M.L.; Khan,T.A.; Mosmann,T.R.. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 1990;248:1230-1234. (Link)

Spits,H.; de Waal,Malefyt R.. Functional characterization of human IL-. Int.Arch.Allergy Immunol. 1992;99:8-15. (Link)

Kullberg,M.C.; Pearce,E.J.; Hieny,S.E.; Sher,A.; Berzofsky,J.A.. Infection with Schistosoma mansoni alters Th1/Th2 cytokine responses to a non-parasite antigen. J.Immunol. 1992;148:3264-3270. (Link)

de Waal,Malefyt R.; Abrams,J.; Bennett,B.; Figdor,C.G.; de Vries,J.E.. Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J.Exp.Med. 1991;174:1209-1220. (Link)

Moore,K.W.; O'Garra,A.; de Waal,Malefyt R.; Vieira,P.; Mosmann,T.R.. Interleukin-10. Annu.Rev.Immunol. 1993;11:165-190. (Link)

Shanafelt,A.B.; Miyajima,A.; Kitamura,T.; Kastelein,R.A.. The amino-terminal helix of GM-CSF and IL-5 governs high affinity binding to their receptors. EMBO J. 1991;10:4105-4112. (Link)

Baer,R.; Bankier,A.T.; Biggin,M.D.; Deininger,P.L.; Farrell,P.J.; Gibson,T.J.; Hatfull,G.; Hudson,G.S.; Satchwell,S.C.; Seguin,C.; .. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 1984;310:207-211. (Link)

Silva,J.S.; Morrissey,P.J.; Grabstein,K.H.; Mohler,K.M.; Anderson,D.; Reed,S.G.. Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection. J.Exp.Med. 1992;175:169-174. (Link)

Bloom,B.R.; Mehra,V.. Immunological unresponsiveness in leprosy. Immunol.Rev. 1984;80:5-28. (Link)