Rat IL-1 alpha Platinum ELISA

Also known as: Hematopoietin 1, IL1F1, Interleukin 1 alpha

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS627*

Cat. No. Size
BMS627 96 tests
BMS627TEN 10 x 96 tests
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Data for Rat IL-1 alpha Platinum ELISA.

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  • Data for Rat IL-1 alpha Platinum ELISA.
Description

Description: The rat IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-1alpha. The rat IL-1alpha ELISA is for research use only. Not for diagnostic or therapeutic procedures.

The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.

Details
Reactivity Rat
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum
Sensitivity 4.0 pg/mL
Standard Curve Range 15.6 - 1,000 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to rat IL-1alpha
Biotin-Conjugate anti-rat IL-1alpha polyclonal antibody
Streptavidin-HRP
Rat IL-1alpha Standard lyophilized, 2 ng/ml upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

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Lee,S.H.; Brennan,F.R.; Jacobs,J.J.; Urban,R.M.; Ragasa,D.R.; Glant,T.T.. Human monocyte/macrophage response to cobalt-chromium corrosion products and titanium particles in patients with total joint replacements. J.Orthop.Res. 1997;15:40-49. (Link)

Yamashita,U.; Shirakawa,F.; Nakamura,H.. Production of interleukin 1 by adult T cell leukemia (ATL) cell lines. J.Immunol. 1987;138:3284-3289. (Link)

Basso,D.; Plebani,M.; Fogar,P.; Panozzo,M.P.; Meggiato,T.; De,Paoli M.; Del,Favero G.. Insulin-like growth factor-I, interleukin-1 alpha and beta in pancreatic cancer: role in tumor invasiveness and associated diabetes. Int J.Clin.Lab Res. 1995;25:40-43. (Link)

Mantovani,G.; Maccio,A.; Bianchi,A.; Curreli,L.; Ghiani,M.; Santona,M.C.; Del Giacco,G.S.. Megestrol acetate in neoplastic anorexia/cachexia: clinical evaluation and comparison with cytokine levels in patients with head and neck carcinoma treated with neoadjuvant chemotherapy. Int J.Clin.Lab Res. 1995;25:135-141. (Link)

Wilmott,R.W.; Frenzke,M.; Kociela,V.; Peng,L.. Plasma interleukin-1 alpha and beta, tumor necrosis factor-alpha, and lipopolysaccharide concentrations during pulmonary exacerbations of cystic fibrosis. Pediatr.Pulmonol. 1994;18:21-27. (Link)

Jurincic-Winkler,C.D.; Gallati,H.; varez-Mon,M.; Sippel,J.; Carballido,J.; Klippel,K.F.. Urinary interleukin-1 alpha levels are increased by intravesical instillation with keyhole limpet hemocyanin in patients with superficial transitional cell carcinoma of the bladder. Eur.Urol. 1995;28:334-339. (Link)

Shanbhag,A.S.; Jacobs,J.J.; Black,J.; Galante,J.O.; Glant,T.T.. Cellular mediators secreted by interfacial membranes obtained at revision total hip arthroplasty. J.Arthroplasty 1995;10:498-506. (Link)

Mizel,S.B.. Interleukin 1 and T cell activation. Immunol.Rev. 1982;63:51-72. (Link)

Yoshida,A.; Asaga,T.; Masuzawa,C.; Kawahara,S.; Yanoma,S.; Harada,M.; Okamoto,T.. Production of cytokines by thyroid carcinoma cell lines. J.Surg.Oncol. 1994;55:104-107. (Link)

Schuster,A.; Haarmann,A.; Wahn,V.. Cytokines in neutrophil-dominated airway inflammation in patients with cystic fibrosis. Eur.Arch.Otorhinolaryngol. 1995;252 Suppl :S59-S60. (Link)

Wood,D.D.; Bayne,E.K.; Goldring,M.B.; Gowen,M.; Hamerman,D.; Humes,J.L.; Ihrie,E.J.; Lipsky,P.E.; Staruch,M.J.. The four biochemically distinct species of human interleukin 1 all exhibit similar biologic activities. J.Immunol. 1985;134:895-903. (Link)