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Anti-Rat CD3 Purified

Also known as: T3

Clone: eBioG4.18 (G4.18)

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 14-0030

Cat. No. Size
14-0030-81 50 ug
14-0030-82 100 ug
14-0030-85 500 ug

Data for Anti-Rat CD3 Purified.

Staining of rat splenocytes with 0.25 ug of Mouse IgG3 Isotype Control Purified (cat. 14-4742) (open...View More

  • Data for Anti-Rat CD3 Purified.
Description

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Details
Host Mouse
Isotype IgG3, kappa
Reactivity Rat
Conjugate Purified
Reported Applications Flow Cytometric Analysis, Immunohistochemical Staining, Immunoprecipitation, Western Blotting
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
17-0030 Anti-Rat CD3 APC 633 - 647 nm 660 nm FC RUO
16-0030 Anti-Rat CD3 Functional Grade Purified FC, IHC, FA RUO
11-0030 Anti-Rat CD3 FITC 488 nm 520 nm FC RUO
12-0030 Anti-Rat CD3 PE 488 - 561 nm 578 nm FC RUO
13-0030 Anti-Rat CD3 Biotin FC, IHC, IHC-F RUO
46-0030 Anti-Rat CD3 PerCP-eFluor® 710 488 nm 710 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
13-4013 Anti-Mouse IgG Biotin FC, ELISA, IHC, WB RUO
17-4317 Streptavidin APC 633 - 647 nm 660 nm FC RUO
14-4742 Mouse IgG3 Isotype Control Purified FC, ICC, IHC RUO
References

References: Tran GT, Carter N, He XY, Spicer TS, Plain KM, Nicolls M, Hall BM, Hodgkinson SJ. Reversal of experimental allergic encephalomyelitis with non-mitogenic, non-depleting anti-CD3 mAb therapy with a preferential effect on T(h)1 cells that is augmented by IL-4. Int Immunol. 2001 Sep;13(9):1109-20. (G4.18, FC, FA, PubMed)

Brenan M, Rees DJ. Sequence analysis or rat integrin alphaE1 and alphaE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 2000 Jun;30(6):1527-37.

Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion.Immunology. 1995 Jan;84(1):142-7.

Nicolls MR, Aversa GG, Pearce NW, Spinelli A, Berger MF, Gurley KE, Hall BM. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.Transplantation. 1993 Mar;55(3):459-68. (G4.18, FC, IP, FA, PubMed)


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