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Anti-Rat CD3 APC

Also known as: T3

Clone: eBioG4.18 (G4.18)

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 17-0030

Cat. No. Size
17-0030-80 25 ug
17-0030-82 100 ug

Data for Anti-Rat CD3 APC.

Staining of rat splenocytes with Anti-Rat CD45R (B220) FITC (cat. 11-0460) and 0.125 ug of Anti-Rat...View More

  • Data for Anti-Rat CD3 APC.
Description

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. \i{in vitro\i}, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. \i{in vivo\i}, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Details
Host Mouse
Isotype IgG3, kappa
Reactivity Rat
Conjugate APC
Laser Red Laser
Emit 660 nm
Excite 633 - 647 nm
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
16-0030 Anti-Rat CD3 Functional Grade Purified FC, IHC, FA RUO
12-0030 Anti-Rat CD3 PE 488 - 561 nm 578 nm FC RUO
13-0030 Anti-Rat CD3 Biotin FC, IHC, IHC-F RUO
11-0030 Anti-Rat CD3 FITC 488 nm 520 nm FC RUO
14-0030 Anti-Rat CD3 Purified FC, IHC, IP, WB RUO
46-0030 Anti-Rat CD3 PerCP-eFluor® 710 488 nm 710 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-4222 Flow Cytometry Staining Buffer FC GPR
25-0084 Anti-Rat CD8a PE-Cy7 488 - 561 nm 775 nm FC RUO
11-0460 Anti-Rat CD45R (B220) FITC 488 nm 520 nm FC, IHC, IHC-F RUO
46-0040 Anti-Rat CD4 PerCP-eFluor® 710 488 nm 710 nm FC RUO
References

References: Tran GT, Carter N, He XY, Spicer TS, Plain KM, Nicolls M, Hall BM, Hodgkinson SJ. Reversal of experimental allergic encephalomyelitis with non-mitogenic, non-depleting anti-CD3 mAb therapy with a preferential effect on T(h)1 cells that is augmented by IL-4. Int Immunol. 2001 Sep;13(9):1109-20. (G4.18, FC, FA, PubMed)

Brenan M, Rees DJ. Sequence analysis or rat integrin alphaE1 and alphaE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 2000 Jun;30(6):1527-37.

Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion.Immunology. 1995 Jan;84(1):142-7.

Nicolls MR, Aversa GG, Pearce NW, Spinelli A, Berger MF, Gurley KE, Hall BM. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.Transplantation. 1993 Mar;55(3):459-68. (G4.18, FC, IP, FA, PubMed)


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