Rabbit TrueBlot® Set (with IP beads)

Description: Rabbit IgG TrueBlot® is a unique horseradish peroxidase conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/WB data with Rabbit IgG TrueBlot, simply substitute the conventional HRP anti-rabbit IgG blotting reagent with Rabbit IgG TrueBlot™ and follow the prescribed protocol for sample preparation and immunoblotting.

Note that there are three key procedural considerations:
1. Protein A or G should not be used for the immunoprecipitation. For immunoprecipitation, anti-mouse IgG beads, anti-rat IgG beads, or anti-rabbit IgG beads should be used for mouse, rat, or rabbit immunoprecipitating antibodies, respectively.
2. Immunoprecipitate should be completely reduced.
3. Milk should be used as the blocking protein for the immunoblot.

References: Kong, D., L. Xu, Y. Yu, W. Zhu, D.W. Andrews, Y. Yoon, and T.H. Kuo. 2005. Regulation of Ca2+-induced permeability transition by BCL-2 is antagonized by Drp1 and hFis1. Molecular and Cellular Biochemistry. 272: 187-199. (Rabbit IgG TrueBlot, PubMed)

DiPerna, G., J. Stack, A.G. Bowie, A. Boyd, G. Kotwal, Z. Zhang, S. Arvikar, E. Latz, K.A. Fitzgerald, and W.L. Marshall. 2004. Poxvirus protein N1L targets the I-kappaB Kinase complex, inhibits signaling to NF-kappaB by the Tumor Necrosis Factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by Toll-like Receptors. J. Biol. Chem. 279: 36570-36578. (Rabbit IgG TrueBlot, PubMed)

Zhang, X., Y. Ozawa, H. Lee, Y. Wen, T. Tan, B. Wadzinski, and E. Seto. 2005. Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes & Development. 19: 827-839. (Rabbit IgG TrueBlot, PubMed)

Lehtonen, S., E. Lehtonen, K. Kudlicka, H. Holthöfer, and M.G. Farquhar. 2004. Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin. Am J Pathol. 165:923-936. (Rabbit IgG TrueBlot, PubMed)

Tyagi A, Agarwal C, Harrison G, Glode LM, Agarwal R. 2004. Silibinin causes cell cycle arrest and apoptosis in human bladder transitional cell carcinoma cells by regulating CDKI-CDK-cyclin cascade, and caspase 3 and PARP cleavages. Carcinogenesis. 25: 1711-20. (Mouse IgG TrueBlot, PubMed)

Okoshi Y, Tahara-Hanaoka S, Nakahashi C, Honda S, Miyamoto A, Kojima H, Nagasawa T, Shibuya K, Shibuya A. 2005. Requirement of the tyrosines at residues 258 and 270 of MAIR-I in inhibitory effect on degranulation from basophilic leukemia RBL-2H3. Int Immunol. 17(1):65-72. (Mouse IgG TrueBlot, PubMed)

Murray, J., M.F. Marusich, R.A. Capaldi, and R. Aggeler. 2004. Focused proteomics: Monoclonal antibody-based isolation of the oxidative phosphorylation machinery and detection of phosphoproteins using a fluorescent phosphoprotein gel stain. Electrophoresis. 25:2520-2525. (Mouse IgG TrueBlot, PubMed)

Hamdane, M., A. Bretteville, A. Sambo, K. Schindowski, S. Begard, A. Delacourte, P. Bertrand, and L. Buee. 2005. p25/Cdk5-mediated retinoblastoma phosphorylation is an early event in neuronal cell death. Journal of Cell Science. 118: 1291-1298. (Rabbit and Mouse IgG TrueBlot, PubMed)