Ready-Set-Go!® ELISPOT Sets
Evaluate cytokine production of individual cells
ELISPOT enables the analysis of activated or responding cells at the single cell level. It is widely used for identification and enumeration of cytokine-producing cells. Cytokines secreted by activated cells are captured locally by the coated antibody on the PVDF membrane and detected using a biotin-conjugated secondary antibody and visualized using avidin-HRP and a precipitating substrate. The colored end product (spot) reveals an individual cytokine-producing cell. Spots can be visualized manually (microscopy) or using an automated reader to capture the microwell images to analyze spot number and size.
Advantages of using Ready-Set-Go! ELISPOT Sets
- Qualitative – visualize individual cytokine-producing cells by microscopy.
- Quantitative – enumerate activated, cytokine-secreting cells in 96-well plates, ideal for automation.
- Exquisite Sensitivity – greater than conventional ELISA, with limits of detection < 1/100,000 protein-secreting cells.