High Sensitivity ELISA Kits

Low abundance target analysis made easy

High Sensitivity ELISAs from eBioscience provide a solution for difficult to detect, low expressing proteins from serum, plasma, or cell culture samples. Improved sensitivity is made possible by the inherent design of further enhancing the signal obtained from conventional HRP-based ELISA. The signal amplification step using biotinylated Tyramid results in an increased sensitivity without loss of resolution or increase in background.

ELISA’s denoted by "extra sensitive" are high sensitive due to their unique matched antibody properties and do not require Signal Amplification.

Advantages of High Sensitivity ELISA Kits

Sensitive – Detect low cytokine concentrations ≤ 1.0 pg/mL for reliable quantification.
Low Sample Volume – 50 μL sample volume required. Ideal for limited sample resources.
Ease-of-Use – Straightforward protocol with all required reagents included.
Use Existing Instrumentation – Standard colorimetric plate reader with absorption measurement at 450 nm.

Learn More About High Sensitivity ELISA Kits

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Name	Sensitivity	Std. Curve Range	Sample Volume	Cat. No.	Reg.
Human sCD40L Platinum ELISA (extra sensitive)	0.01 ng/mL	0.08 - 5.0 ng/mL	20 - 50 uL	BMS293*	RUO
Human sICAM-1 Platinum ELISA (extra sensitive)	0.33 ng/mL	0.63 - 10.0 ng/mL	100 uL (1:100 prediluted)	BMS241*	RUO
Human IFN-gamma High Sensitivity ELISA	0.06 pg/mL	0.16 - 10.0 pg/mL	50 uL	BMS228HS*	RUO
Mouse IFN gamma Platinum ELISA (extra sensitive)	0.09 pg/mL	0.63 - 40 pg/mL	20 uL	BMS609*	RUO
Human IL-1beta High Sensitivity ELISA	0.05 pg/mL	0.16 - 10.0 pg/mL	50 uL	BMS224HS*	RUO
Human IL-2 High Sensitivity ELISA	0.4 pg/mL	0.94 - 60.0 pg/mL	50 uL	BMS221HS*	RUO
Mouse IL-2 High Sensitivity Platinum ELISA	0.05 pg/mL	0.78 - 50 pg/mL	50 uL	BMS601HS*	RUO
Human IL-4 High Sensitivity ELISA	0.1 pg/mL	0.25 - 16.0 pg/mL	50 uL	BMS225HS*	RUO
Mouse IL-4 High Sensitivity ELISA	0.32 pg/mL	0.63 -40 pg/mL	50 æL	BMS613HS*	RUO
Human IL-6 High Sensitivity ELISA	0.03 pg/mL	0.08 - 5.0 pg/mL	50 uL	BMS213HS*	RUO
Mouse IL-6 High Sensitivity ELISA	0.21 pg/mL	1.56 - 100 pg/mL	50 uL	BMS603HS*	RUO
Human IL-10 High Sensitivity ELISA	0.05 pg/mL	0.39 - 25.0 pg/mL	50 uL	BMS215HS*	RUO
Human IL-12p70 High Sensitivity ELISA	0.1 pg/mL	0.16 - 10.0 pg/mL	50 uL	BMS238HS*	RUO
Human IL-17A High Sensitivity ELISA	0.01 pg/mL	0.23 - 15 pg/mL	50 uL	BMS2017HS*	RUO
Human TNF-alpha High Sensitivity ELISA	0.13 pg/mL	0.31 - 20.0 pg/mL	50 uL	BMS223HS*	RUO
Mouse TNF-alpha High Sensitivity ELISA	0.75 pg/ml	3.13-200 pg/ml	50 µl	BMS607HS*	RUO

* = Please click on product link to see all available sizes and pricing.

FC = Flow Cytometry, Intracellular Staining/Flow Cytometry; ELISA = ELISA, ELISPOT, Multiplexing Immunoassays; ICC = Immunocytochemistry; IHC = Immunohistochemistry, Immunofluorescence, Microscopy, Imaging, In Vivo Imaging; FA = Functional Assays, Bioassays, Neutralization, Depletion Studies, Biomolecule Conjugation; WB = Immunoprecipitation, Western Blotting

RUO = Research Use Only; GPR = General Purpose Reagent; ASR = Analyte Specific Reagent. Analytical and performance characteristics are not established; CE = CE-marked reagents