Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human STAT1 (Tyr701) in cell lysates. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately two hours. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
NOTE: phospho-STAT1 requires a 2 hour incubation for optimal assay performance as opposed to the 1 hour needed for any of the InstantOne ELISAs including STAT1 (total)
STAT1 is a member of the Signal Transducers and Activators of Transcription family of transcription factors that are activated via phosphorylation by JAK tyrosine kinases in response to various stimuli, including cytokines and growth factors. More specifically, numerous studies have demonstrated the involvement of STAT1 in controlling gene expression in response to interferon (IFN) stimulation. Depending on the stimulus, tyrosine phosphorylation leads to STAT1 homodimerization (e.g., IFNg) or heterodimerization with STAT2 (e.g., IFNa and IFNb) via its SH2 domains. After dimerization, STAT1 translocates into the nucleus to regulate transcription. Research shows that STAT1 binds interferon-stimulated response elements within the promoters of genes involved in inducing the cellular antiviral response.