Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human NFkB p65 in cell lysates. Detection of mouse and rat NFkB is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.
Nuclear Factor kappa B (NFkB) constitutes a family of transcription factors consisting of five members, including Rel (c-Rel), RelA (p65), RelB, NFkB1 (p50 and its precursor p105), and NFkB2 (p52 and its precursor p100). NFkB members can exist as either homo- or heterodimers. NFkB dimers containing p65 are activators of transcription. In unstimulated cells, NFkB is inactive and retained in the cytoplasm due to binding by inhibitory IkB proteins. Upon stimulation by inducers such as TNFa, IL-1, or PMA, IkBa is phosphorylated and degraded. This results in the release of the NFkB complex from the IKK complex and cleavage of the p105 subunit into its active p50 form. Subsequently, the p50/p65 complex translocates to the nucleus where it activates transcription. NFkB regulates the expression of numerous genes, including IkBa, cytokines, chemokines, adhesion targets, and acute phase proteins involved in growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters.