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phospho-AKT 1/2/3 (Ser473) InstantOne™ ELISA

Also known as: PKB, Protein Kinase B, RAC-PK-alpha

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 85-86042

Cat. No. Size
85-86042-11 96 tests
85-86042-103 10 x 384 tests
85-86042-253 25 x 384 tests
85-86042-503 50 x 384 tests

Data for phospho-AKT 1/2/3 (Ser473) InstantOne™ ELISA.

MCF7 cells were pre-treated with 10 uM wortmannin washed and unstimulated (-) or stimulated with 10...View More

  • Data for phospho-AKT 1/2/3 (Ser473) InstantOne™ ELISA.

Description: The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster AKT 1/2/3 in cell lysates. Detection of rat AKT 1/2/3 is expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.

Reactivity Hamster, Human, Mouse
Components User Manual
InstantOne™ Assay Plate (Part No. IOAP96) - 96 well (sealed)
Cell Lysis Buffer (5X) (Part No. IOCLB1) - 8 mL
Enhancer Solution (Part No. IOES1) - 0.5 mL
Wash Buffer (10X) (Part No. IOWB1) - 8 mL
phospho-AKT 1/2/3 (Ser473) Capture Antibody Reagent (Part No. IOPAKTACAR1) - 3 mL
phospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent (Part No. IOPAKTADAR1) - 3 mL
Positive Control Cell Lysate (lyophilized) (Part No. IOAKTAPC1) - 1 ea
Detection Reagent (TMB Substrate) (Part No. IODR1) - 10 mL
Stop Solution (Part No. IOSS1) - 10 mL
Plate seal - 1 ea
Reported Applications ELISA
TDS Link Download TDS
User Manual Download User Manual
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