Anti-Myosin Heavy Chain Alexa Fluor® 488

Also known as: Myosin II, Conventional myosin

Clone: MF20

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-6503

Cat. No.	Size
53-6503-80	25 ug
53-6503-82	100 ug
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Immunocytochemistry of differentiated C2C12 cells using 5 ug/mL of Mouse IgG2b Isotype Control Alexa...View More

Description

Description: This MF20 monoclonal antibody recognizes the heavy chain of myosin II, specificially the light meromyosin portion, in cardiac and skeletal muscle of vertebrates. Myosin II is composed of two heavy chains and four light chains. The 220-kDa myosin heavy chain exists as four different isoforms due to alternative splicing. Myosins interact with actin and hydrolyze ATP to function in muscle contraction, cytokinesis, and phagocytosis.

The MF20 has been shown to react to myosin from a variety of mammalian, avian and amphibian species, including rat, mouse, human, chicken, zebrafish, and dog.

Details

Host	Mouse
Isotype	IgG2b, kappa
Reactivity	Bovine, Canine, Chicken, Chimpanzee, Guinea Pig, Human, Mouse, Rabbit, Rat
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Immunocytochemistry

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-6503	Anti-Myosin Heavy Chain Purified			ICC, WB	RUO
50-6503	Anti-Myosin Heavy Chain eFluor® 660	633 - 647 nm	668 nm	ICC, IHC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
53-4031	Rat IgG2b K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO
53-4732	Mouse IgG2b K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Wang Z, Wang DZ, Pipes GC, Olson EN. Myocardin is a master regulator of smooth muscle gene expression. Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7129-34. (MF20, ICC)

Sellers JR. Myosins: a diverse superfamily. Biochim Biophys Acta. 2000 Mar 17;1496(1):3-22. Review

Bader D, Masaki T, Fischman DA. Immunochemical analysis of myosin heavy chain during avian myogenesis in vivo and in vitro. J Cell Biol. 1982 Dec;95(3):763-70. (MF20, ICC, WB)

View More Products

Antigens	Area of Biology	Formats
Myosin	Cell Structure	Alexa Fluor 488