Anti-Mouse V beta 9 TCR eFluor® 450

Description: This MR10-2 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 9 chain. Composed of an a and ß chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR10-2 antibody recognizes the V beta 9 chain on T cells from mouse strains with the b haplotype, including BALB/c, CBA/Ca, C3H/He, and C57BL. This gene is deleted in mice bearing the a (e.g., C57BR, C57L, SJL, and SWR) or c (e.g, RIII) haplotypes. T cells expressing V beta 9 are also deleted in mice expressing the minor lymphocyte stimulating (MIs)-1a antigen.

The MR10-2 antibody has been reported to activate V beta 9+ T cells.

References: Joshi PS, Liu JQ, Wang Y, Chang X, Richards J, Assarsson E, Shi FD, Ljunggren HG, Bai XF. Cytokine-induced killer T cells kill immature dendritic cells by TCR-independent and perforin-dependent mechanisms. J Leukoc Biol. 2006 Dec;80(6):1345-53. (MR10-2, FC)

Kanagawa O, Shimizu J, Vaupel BA. Thymic and postthymic regulation of diabetogenic CD8 T cell development in TCR transgenic nonobese diabetic (NOD) mice. J Immunol. 2000 May 15;164(10):5466-73. MR10-2, FC)

Utsunomiya Y, Kosaka H, Kanagawa O. Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo. Eur J Immunol. 1991 Apr;21(4):1007-11. (MR10-2, FC, FA (activating))