Anti-Mouse V beta 12 TCR PerCP-eFluor® 710

Description: This MR11-1 monoclonal antibody reacts with the mouse T cell receptor (TCR) V beta 12 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The MR11-1 antibody recognizes the V beta 12 chain on T cells from mouse strains with the b haplotype, including C57BL/6, B10, and C58/J. V beta 12+ T cells are absent in strains with the a (e.g., SJL) or c (e.g., RIII) haplotypes of the Tcrb gene complex. Finally, mouse strains expressing the minor lymphocyte stimulatory (Mls) antigens, such as BALB/c and C3H, express fewer V beta 12+ T cells.

References: Davalos-Misslitz AC, Worbs T, Willenzon S, Bernhardt G, Förster R. Impaired responsiveness to T-cell receptor stimulation and defective negative selection of thymocytes in CCR7-deficient mice. Blood. 2007 Dec 15;110(13):4351-9. (MR11-1, FC)

Joshi PS, Liu JQ, Wang Y, Chang X, Richards J, Assarsson E, Shi FD, Ljunggren HG, Bai XF. Cytokine-induced killer T cells kill immature dendritic cells by TCR-independent and perforin-dependent mechanisms. J Leukoc Biol. 2006 Dec;80(6):1345-53. (MR11-1, FC)

Abe R, Kanagawa O, Sheard MA, Malissen B, Foo-Phillips M. Characterization of a new minor lymphocyte stimulatory system. I. Cluster of self antigens recognized by "I-E-reactive" V beta s, V beta 5, V beta 11, and V beta 12 T cell receptors for antigen. J Immunol. 1991 Aug 1;147(3):739-49. (MR11-1)

Vacchio MS, Hodes RJ. Selective decreases in T cell receptor V beta expression. Decreased expression of specific V beta families is associated with expression of multiple MHC and non-MHC gene products. J Exp Med. 1989 Oct 1;170(4):1335-46.