Anti-Mouse V beta 11 TCR PerCP-eFluor® 710

Description: This RR3-15 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V beta 11 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-15 antibody recognizes the V beta 11 chain on T cells from mouse strains with the b haplotype in the Tcrb gene complex, including C57BL/6 and B10, but is absent in mice with the a haplotype, such as C57L, SJL, and SWR. In addition, reports indicate that Vbeta11+ T cells are eliminated in mice bearing the MHC Class II I-E molecule. Finally, V beta 11+CD4+ T cells have been associated with graft-versus-host disease (GVHD) severity.

The RR3-15 antibody has been reported to activate V beta 11+ T cells.

References: Domingues HS, Mues M, Lassmann H, Wekerle H, Krishnamoorthy G. Functional and pathogenic differences of Th1 and Th17 cells in experimental autoimmune encephalomyelitis. PLoS One. 2010 Nov 29;5(11):e15531. (RR3-15, FC)

DiRienzo CG, Murphy GF, Friedman TM, Korngold R. T-cell receptor V(alpha) usage by effector CD4+Vbeta11+ T cells mediating graft-versus-host disease directed to minor histocompatibility antigens. Biol Blood Marrow Transplant. 2007 Mar;13(3):265-76.

Bill J, Kanagawa O, Woodland DL, Palmer E. The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells. J Exp Med. 1989 Apr 1;169(4):1405-19. (RR3-15)