Anti-Mouse V alpha 3.2 TCR PerCP-eFluor® 710

Description: This RR3-16 monoclonal antibody reacts with the mouse T cell receptor (TCR) V alpha 3.2 chain. Composed of an alpha and beta chain, TCR specificity is typically determined by Va, Ja, Vb, Db, and Jb gene rearrangement. The RR3-16 antibody recognizes the V alpha 3.2 chain on T cells from mouse strains bearing the b (e.g., C57BL/6) or c haplotype (e.g., SJL, SWR, and NOD) in the Tcra gene complex. The V alpha 3.2 chain is absent in mice with the a (e.g., Balb/c, AKR, C3H) and d (e.g., DBA/1 and DBA/2) haplotypes. Studies demonstrate that the V alpha 3.2 TCR is more highly expressed on CD8+ T cells.

References: Richman SA, Aggen DH, Dossett ML, Donermeyer DL, Allen PM, Greenberg PD, Kranz DM. Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain ValphaVbeta fragments. Mol Immunol. 2009 Feb;46(5):902-16. (RR3-16, FC)

Utsunomiya Y, Bill J, Palmer E, Gollob K, Takagaki Y, Kanagawa O. Analysis of a monoclonal rat antibody directed to the alpha-chain variable region (V alpha 3) of the mouse T cell antigen receptor. J Immunol. 1989 Oct 15;143(8):2602-8. (RR3-16, FC)