Anti-Mouse TNF alpha Cy5

Also known as: Tumor Necrosis Factor alpha

Clone: MP6-XT22

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 19-7321

Cat. No.	Size
19-7321-81	50 ug
19-7321-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Intracellular staining of Mouse Cytokine Positive Control Cells (cat. 00-4500) with Anti-Mouse CD4...View More

Description

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Details

Host	Rat
Isotype	IgG1, kappa
Reactivity	Mouse
Conjugate	Cy5
Laser	Red Laser
Emit	670 nm
Excite	633 - 647 nm
Legal	FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
11-7321	Anti-Mouse TNF alpha FITC	488 nm	518 nm	FC	RUO
17-7321	Anti-Mouse TNF alpha APC	633 - 647 nm	660 nm	FC	RUO
12-7321	Anti-Mouse TNF alpha PE	488 - 561 nm	575 nm	FC	RUO
48-7321	Anti-Mouse TNF alpha eFluor® 450	405 nm	450 nm	FC	RUO
53-7321	Anti-Mouse TNF alpha Alexa Fluor® 488	488 nm	519 nm	FC	RUO
14-7321	Anti-Mouse TNF alpha Purified			FC, IHC	RUO
25-7321	Anti-Mouse TNF alpha PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
46-7321	Anti-Mouse TNF alpha PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
16-7321	Anti-Mouse TNF alpha Functional Grade Purified			FA	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4500	Mouse Cytokine Positive Control Cells			FC	RUO
11-0041	Anti-Mouse CD4 FITC	488 nm	518 nm	FC	RUO
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Chackerian B, Lowy DR and Schiller JT. Conjugation of a self-antigen to papillomavirus-like particles allows for efficient induction of protective autoantibodies. J Clin Invest. 2001. 108(3):415-23. (IHC frozen, PubMed)

Williams RO, Mauri C, et al.Therapeutic actions of cyclosporine and anti-tumor necrosis factor alpha in collagen-induced arthritis and the effect of combination therapy. Arthritis Rheum. 1998. 41(10):1806-12. (IHC frozen, PubMed)

Hunter CA, Litton MJ, et al. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 1994. 170(4): 939-45.

Litton MJ, Sander B, et al. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods 1994. 175(1): 47-58.

Abrams JS, Roncarolo MG, et al. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992. 127: 5-24.

View More Products

Antigens	Area of Biology	Cell Type	Formats
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells	Cy5