Anti-Mouse TNF alpha Alexa Fluor® 488

Also known as: Tumor Necrosis Factor alpha

Clone: MP6-XT22

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-7321

Cat. No.	Size
53-7321-80	25 ug
53-7321-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Intracellular staining of restimulated C57BL/6 splenocytes with staining buffer (autofluorescence)...View More

Description

Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+)T cells. TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Details

Host	Rat
Isotype	IgG1, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
17-7321	Anti-Mouse TNF alpha APC	633 - 647 nm	660 nm	FC	RUO
19-7321	Anti-Mouse TNF alpha Cy5	633 - 647 nm	670 nm	FC	RUO
48-7321	Anti-Mouse TNF alpha eFluor® 450	405 nm	450 nm	FC	RUO
12-7321	Anti-Mouse TNF alpha PE	488 - 561 nm	575 nm	FC	RUO
16-7321	Anti-Mouse TNF alpha Functional Grade Purified			FA	RUO
46-7321	Anti-Mouse TNF alpha PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
25-7321	Anti-Mouse TNF alpha PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
14-7321	Anti-Mouse TNF alpha Purified			FC, IHC	RUO
11-7321	Anti-Mouse TNF alpha FITC	488 nm	518 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
53-4301	Rat IgG1 K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Hunter CA, Litton MJ, et al. 1994. Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis. J Infect Dis. 170(4): 939-45.

Litton MJ, Sander B, et al. 1994. Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B. J Immunol Methods 175(1): 47-58.

Abrams JS, Roncarolo MG, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.

Chackerian B, Lowy DR and Schiller JT. 2001. Conjugation of a self-antigen to papillomavirus-like particles allows for efficient induction of protective autoantibodies. J Clin Invest. 108(3):415-23. (IHC frozen, PubMed)

Williams RO, Mauri C, et al. 1998. Therapeutic actions of cyclosporine and anti-tumor necrosis factor alpha in collagen-induced arthritis and the effect of combination therapy. Arthritis Rheum. 41(10):1806-12. (IHC frozen, PubMed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells	Alexa Fluor 488