Anti-Mouse TIGIT Alexa Fluor® 647 (To be Discontinued. Refer to Cat. No. 50-9501)

Also known as: VSTM3, WUCAM

Clone: GIGD7

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: Replacement format is eFluor 660. Select Cat. No. 50-9501.

SKU# 51-9501

Cat. No.	Size
51-9501-80	25 ug
51-9501-82	100 ug
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Staining of sheep red blood cell-immunized C57Bl/6 splenocytes with Anti-Mouse CD3 PerCP-eFluor®...View More

Description

Description: The GIGD7 monoclonal antibody reacts with mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT, VSTM3, WUCAM), a 26 kDa protein that is a member of the poliovirus receptor (PVR) family. In mice, the expression of TIGIT has been reported in follicular T helper cells, while in humans, the expression of TIGIT has been reported on NK cells, regulatory T cells, follicular T helper cells, memory CD4+ T cells, and CD8+ T cells. TIGIT is not expressed on B cells or naïve CD4+ T cells. TIGIT is upregulated on CD4+ T cells following activation. TIGIT can interact with certain members of the PVR and PVR-like families, including CD155, and also mediates the interaction of NK cells and T cells with antigen presenting cells, fibroblasts and endothelial cells that express PVR and PVR-like proteins.

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 - 647 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
12-9501	Anti-Mouse TIGIT PE	488 - 561 nm		FC	RUO
50-9501	Anti-Mouse TIGIT eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC	RUO
46-9501	Anti-Mouse TIGIT PerCP-eFluor® 710	488 nm	710 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
11-9949	Anti-Mouse/Rat CD278 (ICOS) FITC	488 nm	518 nm	FC	RUO
12-9500	Anti-Human TIGIT PE	488 - 561 nm	575 nm	FC	RUO
12-9985	Anti-Mouse CD279 (PD-1) PE	488 - 561 nm	575 nm	FC	RUO
46-0032	Anti-Mouse CD3 PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
48-0042	Anti-Mouse CD4 eFluor® 450	405 nm		FC	RUO
51-4321	Rat IgG2a K Isotype Control Alexa Fluor® 647 (To Be Discontinued. Refer to Cat. No. 50-4321)	633 - 647 nm	668 nm	FC, ICC, IHC	RUO

References

References: Seth S, Ravens I, Kremmer E, Maier MK, Hadis U, Hardtke S, Förster R, Bernhardt G. Abundance of follicular helper T cells in Peyer's patches is modulated by CD155. Eur J Immunol. 2009 Nov;39(11):3160-70.

Stanietsky N, Simic H, Arapovic J, Toporik A, Levy O, Novik A, Levine Z, Beiman M, Dassa L, Achdout H, Stern-Ginossar N, Tsukerman P, Jonjic S, Mandelboim O. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17858-63.

Boles KS, Vermi W, Facchetti F, Fuchs A, Wilson TJ, Diacovo TG, Cella M, Colonna M. A novel molecular interaction for the adhesion of follicular CD4 T cells to follicular DC. Eur J Immunol. 2009 Mar;39(3):695-703.

Yu X, Harden K, Gonzalez LC, Francesco M, Chiang E, Irving B, Tom I, Ivelja S, Refino CJ, Clark H, Eaton D, Grogan JL. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. Nat Immunol. 2009 Jan;10(1):48-57.

View More Products

Antigens	Area of Biology	Cell Type	Formats
TIGIT	Adaptive Immunity	Tfh Cells	Alexa Fluor 647