Description: This Mouse Th1/Th2/Th17/Th22 13plex FlowCytomix Multiplex kit is designed for the measurement of mouse IL-1alpha, IL-2 , IL-4, IL-5, IL-6, IL-10, IL-13, IL-17A, IL-21, IL-22, IL-27, IFN-gamma and TNF-alpha in an immunoassay analyzed on a flow cytometer.
Multiplex kits are designed to focus on a specific group of functionally related proteins and contain all necessary reagents for detection of multiple analyte combinations.
CD4 + T helper cells are critical mediators of the cellular immune response. For many years, due to cytokine expression patterns, it was thought that CD4+ T helper cells existed as a dichotomy of lineages named Type-1 helper cells (Th-1) and type-2 helper cells (Th2). Type-1 cytokines include IL-2, IFN-gamma, IL-12 and TNF-beta, while Type-2 cytokines include IL-4, IL-5, IL-6, IL-10 and IL-13. However, as these Th1 / Th2 subsets were analyzed more closely, it became apparent that the T helper cell population was not limited to these two subsets. Although it has long been appreciated that IL-17 (also known as IL-17A) production by T cells is required for protection against some pathogens, in 2000 it was demonstrated that IL-17A was produced by a unique subset of T helper cells. Subsequently, it was definitively shown that T cells could differentiate into IL-17-producing cells in vitro and in vivo independently of Th1 or Th2 cell development thereby establishing Th17 cells as a unique T helper cell lineage. Functionally, Th17 cells play a role in host defense against extracellular pathogens by mediating the recruitment of neutrophils and macrophages to infected tissues. Moreover, it is becoming evident that aberrant regulation of Th17 cells may play a significant role in the pathogenesis of multiple inflammatory and autoimmune disorders.
