Anti-Human/Mouse TdT PE

Description: This 19-3 monoclonal antibody recognizes human and mouse terminal-deoxynucleotidyl transferase (TdT), a 60 kDa polymerase responsible for the template-independent addition of N-nucleotides at gene segment junctions of developing lymphocytes. Expression is found in the nucleus of immature lymphocytes but not in mature lymphocytes or non-lymphoid cells. Tdt plays a critical role during TCR and Ig gene rearrangement. Regulation of expression in the mouse thymus correlates with T cell selection; decreased or absent after positive selection. Additionally Tdt has been shown to be present in lymphoma or lymphoblastic leukemia cells.

References: Komori T, Okada A, Stewart V, Alt FW. Lack of N Regions in Antigen Receptor Variable Region Genes of TdT-Deficient Lymphocytes. Science 261.5125 (1993): 1171-1175.

Bogue M, Gilfillan S, Benoist C, Mathis D. Regulation of N-region diversity in antigen receptors through thymocyte differentiation and thymus ontogeny. Proc Natl Acad Sci U S A. 1992 Nov 15;89(22):11011-5.

Bardales RH, Carrato A, Fleischer M, Schwartz MK, Koziner B. Detection of terminal deoxynucleotidyl transferase (TdT) by flow cytometry in leukemic disorders. J Histochem Cytochem. 1989 Apr;37(4):509-13.

Bollum FJ, Chang LM. Immunological detection of a conserved structure for terminal deoxynucleotidyltransferase. J Biol Chem. 1981 Aug 25;256(16):8767-70.