Anti-Mouse Siglec H Alexa Fluor 647 (Discontinued. Refer to Cat. No. 50-0333)

Also known as: Sialic Acid-Binding Immunoglobulin-Like Lectin H

Clone: eBio440c

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: Replacement format is eFluor 660. Select Cat. No. 50-0333.

SKU# 51-0333

Cat. No.	Size
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Staining of 129 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (cat. 11-0452) and 0.125 ug of...View More

Description

Description: The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Details

Host	Rat
Isotype	IgG2b
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 - 647 nm
Legal	Pat. No.pending. Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
25-0333	Anti-Mouse Siglec H PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
48-0333	Anti-Mouse Siglec H eFluor® 450	405 nm		FC	RUO
16-0333	Anti-Mouse Siglec H Functional Grade Purified			FC, FA	RUO
46-0333	Anti-Mouse Siglec H PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
12-0333	Anti-Mouse Siglec H PE	488 - 561 nm		FC	RUO
50-0333	Anti-Mouse Siglec H eFluor® 660 (Alexa® 647 Replacement)	633 - 647 nm	668 nm	FC	RUO
14-0333	Anti-Mouse Siglec H Purified			FC, IHC	RUO
11-0333	Anti-Mouse Siglec H FITC	488 nm		FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
11-0452	Anti-Human/Mouse CD45R (B220) FITC	488 nm		FC	RUO

References

References: Amanda Blasius, William Vermi, Anne Krug, Fabio Facchetti, Marina Cella, and Marco Colonna. A cell-surface molecule selectively expressed on murine naturalinterferonalpha producing cells that blocks secretion of interferonalpha. Blood. 2004;103:4201-4206 (440c, FC, IH/F, FA PubMed)

Blasius AL, Giurisato E, Cella M, Schreiber RD, Shaw AS, Colonna M. Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation.J Immunol. 2006 Sep 1;177(5):3260-5.(440c, FC, PubMed)

Blasius AL, Cella M, Maldonado J, Takai T, Colonna M. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12.Blood. 2006 Mar 15;107(6):2474-6. Epub 2005 Nov 17.(440c, FC, PubMed)

Kreisel FH, Blasius A, Kreisel D, Colonna M, Cella M. Interferon-producing cells develop from murine CD31(high)/Ly6C(-) marrow progenitors. Cell Immunol. 2006 Aug;242(2):91-8.

View More Products

Antigens	Area of Biology	Cell Type	Formats
Siglec H	Innate Immunity	Dendritic Cells Macrophage & Monocyte Cells	Alexa Fluor 647