Anti-Mouse/Rat TNF alpha PE

Also known as: Tumor Necrosis Factor alpha

Clone: TN3-19

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 12-7423

Cat. No.	Size
12-7423-41	25 tests
12-7423-81	50 ug
12-7423-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail...View More

Description

Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.

Details

Host	Armenian Hamster
Isotype	IgG
Reactivity	Mouse, Rat
Conjugate	PE
Laser	Blue Laser, Green Laser, Yellow-Green Laser
Emit	575 nm
Excite	488 - 561 nm
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
16-7423	Anti-Mouse/Rat TNF alpha Functional Grade Purified			FA	RUO
36-7423	Anti-Mouse/Rat TNF alpha Functional Grade Biotin			FA	RUO
50-7423	Anti-Mouse/Rat TNF alpha eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
11-7423	Anti-Mouse/Rat TNF alpha FITC	488 nm	518 nm	FC	RUO
25-7423	Anti-Mouse/Rat TNF alpha PE-Cyanine7	488 - 561 nm	775 nm	FC, ELISA, FA	RUO
14-7423	Anti-Mouse/Rat TNF alpha Purified			FC, ELISA, FA	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4975	Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)			FC, FA	GPR
12-4888	Armenian Hamster IgG Isotype Control PE	488 - 561 nm		FC	RUO
48-0041	Anti-Mouse CD4 eFluor® 450	405 nm	450 nm	FC	RUO
65-0865	Fixable Viability Dye eFluor® 780			FC	GPR
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Finkelman, F.D., and S.C. Morris. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 1999. 11: 1811-1818.

Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.

Takahashi S, Kapas L, Fang J, Krueger JM. An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits. Brain Res. 1995. 690: 241-44.

Rabinovici R, Bugelski PJ, Esser KM, Hillegass LM, Griswold DE, Vernick J, Feuerstein G.Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats. Macol Exp Ther. 1993. 267: 1550-57.

Merrick BA, He CY, Craig WA, Clark GC, Corsini E, Rosenthal GJ, Mansfield BK, Selkirk JK. Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment. Appl Theor Electrophor. 1992. 2: 177-87.

Sheehan KC, Ruddle NH, Schreiber RD. Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors. J Immunol. 1989. 142: 3884-93.

View More Products

Antigens	Area of Biology	Cell Type	Formats
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells	PE