Anti-Mouse/Rat TNF alpha FITC

Also known as: Tumor Necrosis Factor alpha

Clone: TN3-19

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 11-7423

Cat. No.	Size
11-7423-41	25 tests
11-7423-81	50 ug
11-7423-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail...View More

Description

Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.

Details

Host	Armenian Hamster
Isotype	IgG
Reactivity	Mouse, Rat
Conjugate	FITC
Laser	Blue Laser
Emit	518 nm
Excite	488 nm
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
16-7423	Anti-Mouse/Rat TNF alpha Functional Grade Purified			FA	RUO
25-7423	Anti-Mouse/Rat TNF alpha PE-Cyanine7	488 - 561 nm	775 nm	FC, ELISA, FA	RUO
50-7423	Anti-Mouse/Rat TNF alpha eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
14-7423	Anti-Mouse/Rat TNF alpha Purified			FC, ELISA, FA	RUO
36-7423	Anti-Mouse/Rat TNF alpha Functional Grade Biotin			FA	RUO
12-7423	Anti-Mouse/Rat TNF alpha PE	488 - 561 nm	575 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4975	Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)			FC, FA	GPR
11-4888	Armenian Hamster IgG Isotype Control FITC	488 nm		FC, ICC, IHC	RUO
14-8021	Mouse IL-2 Recombinant Protein			ELISA, FA	RUO
14-8041	Mouse IL-4 Recombinant Protein			ELISA, FA	RUO
16-0032	Anti-Mouse CD3 Functional Grade Purified			FC, FA	RUO
16-0281	Anti-Mouse CD28 Functional Grade Purified			FC, FA	RUO
48-0041	Anti-Mouse CD4 eFluor® 450	405 nm	450 nm	FC	RUO
65-0865	Fixable Viability Dye eFluor® 780			FC	GPR
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Finkelman, F.D., and S.C. Morris. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 1999. 11: 1811-1818.

Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.

Takahashi S, Kapas L, Fang J, Krueger JM. An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits. Brain Res. 1995. 690: 241-44.

Rabinovici R, Bugelski PJ, Esser KM, Hillegass LM, Griswold DE, Vernick J, Feuerstein G.Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats. Macol Exp Ther. 1993. 267: 1550-57.

Merrick BA, He CY, Craig WA, Clark GC, Corsini E, Rosenthal GJ, Mansfield BK, Selkirk JK. Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment. Appl Theor Electrophor. 1992. 2: 177-87.

Sheehan KC, Ruddle NH, Schreiber RD. Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors. J Immunol. 1989. 142: 3884-93.

View More Products

Antigens	Area of Biology	Cell Type	Formats
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors Innate Immunity	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells	FITC