Anti-Mouse/Rat TNF alpha eFluor® 660 (Alexa Fluor® 647 Replacement)

Also known as: Tumor necrosis factor

Clone: TN3-19

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 50-7423

Cat. No.	Size
50-7423-80	25 ug
50-7423-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Mouse splenocytes were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail...View More

Description

Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor alpha (TNF alpha), but not with human TNF alpha. The TN3-19.12 antibody is a neutralizing antibody. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF alpha is expressed as a membrane-bound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF alpha have been observed.

Details

Host	Armenian Hamster
Isotype	IgG
Reactivity	Mouse, Rat
Conjugate	eFluor 660
Laser	Red Laser
Emit	668 nm
Excite	633 nm
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
25-7423	Anti-Mouse/Rat TNF alpha PE-Cyanine7	488 - 561 nm	775 nm	FC, ELISA, FA	RUO
36-7423	Anti-Mouse/Rat TNF alpha Functional Grade Biotin			FA	RUO
14-7423	Anti-Mouse/Rat TNF alpha Purified			FC, ELISA, FA	RUO
12-7423	Anti-Mouse/Rat TNF alpha PE	488 - 561 nm	575 nm	FC	RUO
16-7423	Anti-Mouse/Rat TNF alpha Functional Grade Purified			FA	RUO
11-7423	Anti-Mouse/Rat TNF alpha FITC	488 nm	518 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4975	Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)			FC, FA	GPR
12-0041	Anti-Mouse CD4 PE	488 - 561 nm	575 nm	FC	RUO
65-0865	Fixable Viability Dye eFluor® 780			FC	GPR
88-8823	Intracellular Fixation & Permeabilization Buffer (plus Brefeldin A) (previously named IC Fixation & Permeabilization Buffer)			FC	GPR

References

References: Sheehan KC, Ruddle NH, Schreiber RD. 1989. Generation and characterization of hamster monoclonal antibodies that neutralize murine tumor necrosis factors. J Immunol. 142: 3884-93.

Rabinovici R, Bugelski PJ, Esser KM, Hillegass LM, Griswold DE, Vernick J, Feuerstein G. 1993. Tumor necrosis factor-alpha mediates endotoxin-induced lung injury in platelet activating factor-primed rats. Macol Exp Ther. 267: 1550-57.

Merrick BA, He CY, Craig WA, Clark GC, Corsini E, Rosenthal GJ, Mansfield BK, Selkirk JK. 1992. Two dimensional gel electrophoresis of cellular and secreted proteins from rat alveolar macrophages after lipopolysaccharide treatment. Appl Theor Electrophor. 2: 177-87.

Takahashi S, Kapas L, Fang J, Krueger JM. 1995. An anti-tumor necrosis factor antibody suppresses sleep in rats and rabbits. Brain Res. 690: 241-44.

Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.

Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

View More Products

Antigens	Area of Biology	Cell Type	Formats
TNF alpha	Apoptosis Cytokines, Chemokines, Growth Factors	Macrophage & Monocyte Cells NK & NKT Cells T Cells Th1 Cells	eFluor 660