Anti-Mouse/Rat Nestin Purified

Description: The monoclonal antibody Rat-401 recognizes mouse and rat nestin, a Class VI intermediate filament protein (>170 kDa) primarily expressed in neuroepithelial stem cells. Upon neural differentiation, expression is down-regulated and within the adult nervous system is limited to progenitor cells in the cortical subventricular zone, the hippocampal dentate gyrus, dorsal root ganglia satellite cells, and a subpopulation of Schwann cells. Nestin expression is also present in other progenitor cell populations such as pancreatic islet, angiogenic endothelial cells, and bone marrow mesenchymal stem cells. Nestin filaments require vimentin and desmin for assembly into heterodimers and polymers. Upon differentiation, Nestin-containing filaments are replaced by cell type specific intermediate filament proteins, such as GFAP.

References: Lindsley RC, Gill JG, Kyba M, Murphy TL, Murphy KM. Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development. 2006 Oct;133(19):3787-96 (Rat-401, ICC, PubMed)

Dubé M, Huot ME, Khandjian EW. Muscle specific fragile X related protein 1 isoforms are sequestered in the nucleus of undifferentiated myoblast. BMC Genet. 2000;1:4. (Rat-401)

Hockfield S, McKay RD. Identification of major cell classes in the developing mammalian nervous system.J Neurosci. 1985 Dec;5(12):3310-28.(Rat401, ICC, PubMed)