Anti-Mouse/Rat Foxp3 FITC

Also known as: Forkhead Box P3, Scurfin, JM2

Clone: FJK-16s

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 11-5773

Cat. No. Size
11-5773-82 100 ug
11-5773-80 25 ug
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Data for Anti-Mouse/Rat Foxp3 FITC.

Surface staining of BALB/c splenocytes with Anti-Mouse CD4 PE (cat. 12-0042) and Anti-Mouse CD25 APC...View More

  • Data for Anti-Mouse/Rat Foxp3 FITC.
Description

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Bovine, Canine, Mouse, Pig, Rat
Conjugate FITC
Laser Blue Laser
Excite 488 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis, Immunohistochemical Staining of Frozen Tissue Sections
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
56-5773 Anti-Mouse/Rat Foxp3 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
58-5773 Anti-Mouse/Rat Foxp3 Alexa Fluor® 532 532 nm 561 nm FC RUO
17-5773 Anti-Mouse/Rat Foxp3 APC 633 - 647 nm 660 nm FC RUO
15-5773 Anti-Mouse/Rat Foxp3 PE-Cyanine5 488 - 561 nm 667 nm FC RUO
61-5773 Anti-Mouse/Rat Foxp3 PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
13-5773 Anti-Mouse/Rat Foxp3 Biotin FC, IHC, WB RUO
48-5773 Anti-Mouse/Rat Foxp3 eFluor® 450 405 nm 445 nm FC RUO
14-5773 Anti-Mouse/Rat Foxp3 Purified IHC, WB RUO
IH93-5773 Anti-Mouse/Rat Foxp3 eFluor® 605NC (for IHC/ICC) (Discontinued) 350 - 590 nm 605 nm ICC, IHC RUO
53-5773 Anti-Mouse/Rat Foxp3 Alexa Fluor® 488 488 nm 519 nm FC RUO
12-5773 Anti-Mouse/Rat Foxp3 PE 488 - 561 nm 578 nm FC RUO
42-5773 Anti-Mouse/Rat Foxp3 eFluor® 615 595 nm 615 nm ICC, IHC RUO
IH95-5773 Anti-Mouse/Rat Foxp3 eFluor® 650NC (for IHC/ICC) (Discontinued) 350 - 635 nm 650 nm ICC, IHC RUO
35-5773 Anti-Mouse/Rat Foxp3 PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
25-5773 Anti-Mouse/Rat Foxp3 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
41-5773 Anti-Mouse/Rat Foxp3 eFluor® 570 555 nm 570 nm IHC RUO
50-5773 Anti-Mouse/Rat Foxp3 eFluor® 660 633 - 647 nm 668 nm FC RUO
45-5773 Anti-Mouse/Rat Foxp3 PerCP-Cyanine5.5 488 nm 695 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-5521 Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent FC GPR
11-4321 Rat IgG2a K Isotype Control FITC 488 nm FC, ICC, IHC RUO
17-0251 Anti-Mouse CD25 APC 633 - 647 nm 660 nm FC RUO
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
12-0042 Anti-Mouse CD4 PE 488 - 561 nm 575 nm FC RUO
72-5776 Anti-Human Foxp3 Staining Set PE 488 nm 575 nm FC RUO
References

References: Gerner W, Stadler M, Hammer SE, Klein D, Saalmüller A. Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry. Vet Immunol Immunopathol. 2010 Nov 15;138(1-2):154-8 (FJK-16s, porcine FC, PubMed)

Käser T, Gerner W, Hammer SE, Patzl M, Saalmüller A. Detection of Foxp3 protein expression in porcine T lymphocytes. Vet Immunol Immunopathol. 2008 Sep 15;125(1-2):92-101 (FJK-16s, IC flow porcine, PubMed)

Habicht A, Dada S, Jurewicz M, Fife BT, Yagita H, Azuma M, Sayegh MH, Guleria I. A link between PDL1 and T regulatory cells in fetomaternal tolerance. J Immunol. 2007 Oct 15;179(8):5211-9. (FJK-16s, IH/F, PubMed)

Heimesaat MM, Fischer A, Siegmund B, Kupz A, Niebergall J, Fuchs D, Jahn HK, Freudenberg M, Loddenkemper C, Batra A, Lehr HA, Liesenfeld O, Blaut M, Göbel UB, Schumann RR, Bereswill S. Shift towards pro-inflammatory intestinal bacteria aggravates acute murine colitis via Toll-like receptors 2 and 4. PLoS ONE. 2007 Jul 25;2(7):e662. (FJK-16s, IHC paraffin, PubMed)

Biller BJ, Elmslie RE, Burnett RC, Avery AC, Dow SW. Use of FoxP3 expression to identify regulatory T cells in healthy dogs and dogs with cancer. Vet Immunol Immunopathol. 2007 Mar 15;116(1-2):69-78 (FJK-16s, IC Flow in canine, PubMed)

Leithäuser F, Meinhardt-Krajina T, Fink K, Wotschke B, Möller P, Reimann J. Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis. Am J Pathol. 2006 Jun;168(6):1898-909. (FJK-16s, IHC paraffin, PubMed)

Kohm AP, McMahon JS, Podojil JR, Begolka WS, Degutes M, Kasprowicz DJ, Ziegler SF, Miller SD. Cutting Edge: Anti-CD25 Monoclonal Antibody Injection Results in the Functional Inactivation, Not Depletion, of CD4+CD25+ T Regulatory Cells.J Immunol. 2006 Mar 15;176(6):3301-5. (FJK-16s; intracellular staining and IH/F, PubMed)

Suvas S, Azkur AK, Rouse BT.Qa-1b and CD94-NKG2a interaction regulate cytolytic activity of herpes simplex virus-specific memory CD8+ T cells in the latently infected trigeminal ganglia. J Immunol. 2006 Feb 1;176(3):1703-11. (FJK-16s, Immunofluorescence, PubMed)

Fields ML, Hondowicz BD, Metzgar MH, Nish SA, Wharton GN, Picca CC, Caton AJ, Erikson J. CD4+CD25+ Regulatory T cells inhibit the maturation but not the initiation of an autoantibody response. J Immunol. 2005 Oct 1;175(7):4255-64. (FJK-16s, IC Flow, PubMed)

Ko K, Yamazaki S, Nakamura K, Nishioka T, Hirota K, Yamaguchi T, Shimizu J, Nomura T, Chiba T, Sakaguchi S. Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+CD25+CD4+ regulatory T cells. J Exp Med. 2005 Oct 3;202(7):885-91. (FJK-16s, IC Flow, PubMed)

Aswad F, Kawamura H, Dennert G. High Sensitivity of CD4+CD25+ Regulatory T Cells to Extracellular Metabolites Nicotinamide Adenine Dinucleotide and ATP: A Role for P2X7 Receptors. J Immunol. 2005 Sep 1;175(5):3075-83. (FJK-16s, IC Flow, PubMed)

Beyersdorf N, Gaupp S, Balbach K, Schmidt J, Toyka KV, Lin CH, Hanke T, Hünig T, Kerkau T, Gold R. Selective targeting of regulatory T cells with CD28 superagonists allows effective therapy of experimental autoimmune encephalomyelitis. J Exp Med. 2005 Aug 1;202(3):445-55. (FJK-16s, IC Flow in Rat, PubMed)

Fontenot JD, Rasmussen JP, Williams LM, Dooley JL, Farr AG, Rudensky AY. Regulatory T cell lineage specification by the forkhead transcription factor foxp3. Immunity. 2005 Mar;22(3):329-41.

Hori S, Nomura T, Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003 Feb 14;299(5609):1057-61.


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