Anti-Mouse/Rat 3G11 Sialoganglioside Antigen Purified

Description: The eBio3G11 monoclonal antibody reacts with the mouse 3G11antigen. The 3G11 antibody was isolated as an autoantibody against the 3G11 sialylated carbohydrate antigen, which was found to be expressed by thymoyctes and peripheral T cells, but not by B cells, myeloid cells or erythrocytes. 3G11 is expressed at higher levels on CD4+ T cells than on CD8+ T cells, however expression on both of these subsets decreases with advancing age. In thymus, 3G11 is expressed on approximately 20% of total thymocytes. 3G11 has also been used as a marker to distinguish naïve and memory T cells, as 3G11 is irreversibly down-regulated upon activation of CD4+ T cells. The 3G11 antibody is able to block binding of antibodies to CD3ε, suggesting close proximity of these two proteins.

Recently, it has been demonstrated that loss of 3G11 expression in experimental autoimmune encephalomyelitis (EAE) is associated with an anergic, regulatory T phenotype. Purified 3G11- T cells were able to suppress proliferation and cytokine production of 3G11+ T cells, and part of this effect was due to the secretion of IL-10.

References: Zhao Z, Ciric B, Yu S, Zhang GX, Rostami A. Targeting ganglioside epitope 3G11 on the surface of CD4+ T cells suppresses EAE by altering the Treg/Th17 cell balance. Int Immunol. 2010 Oct;22(10):817-26.

Zhao Z, Ciric B, Yu S, Li H, Yang J, Kamoun M, Zhang GX, Rostami A. Expression of 3G11 epitope defines subpopulations of regulatory T cells with different suppressive potency. J Neurol Sci. 2010 Aug 15;295(1-2):66-74 (3G11, FC, PubMed)

Zhang GX, Yu S, Calida D, Zhao Z, Gran B, Kamoun M, Rostami A. Loss of the surface antigen 3G11 characterizes a distinct population of anergic/regulatory T cells in experimental autoimmune encephalomyelitis. J Immunol. 2006 Mar 15;176(6):3366-73. (3G11, FC, PubMed)

Hayakawa K, Hardy RR. Murine CD4+ T cell subsets defined. J Exp Med. 1988 Nov 1;168(5):1825-38. (3G11, FC, PubMed)

Ernst DN, Hobbs MV, Torbett BE, Glasebrook AL, Rehse MA, Bottomly K, Hayakawa K, Hardy RR, Weigle WO. Differences in the expression profiles of CD45RB, Pgp-1, and 3G11 membrane antigens and in the patterns of lymphokine secretion by splenic CD4+ T cells from young and aged mice. J Immunol. 1990 Sep 1;145(5):1295-302. (3G11, FC, PubMed)