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Mouse PrimeFlow® RNA Assay (3-plex, see replacement product cat. no. 88-18005)

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 88-18001

Cat. No. Size
88-18001-204 40 tests
88-18001-210 100 tests

Data for Mouse PrimeFlow™ RNA Assay.

Mouse splenocytes were unstimulated (left) or stimulated with Anti-Mouse CD3e and CD28 Functional...View More

  • Data for Mouse PrimeFlow™ RNA Assay.
Description

Description: This PrimeFlow® RNA Assay (3-plex) is an in situ hybridization assay that combines the power of branched DNA technology with the single cell resolution of flow cytometry. Branched DNA technology uses pairs of target-specific probes to achieve 8,000-16,000 fold signal amplification from a desired mRNA transcript. This assay allows simultaneous detection of up to three RNA targets in combination with cell surface and intracellular staining using fluorochrome-conjugated antibodies to allow further discrimination of specific cell subpopulations.

This Mouse PrimeFlow RNA Assay kit contains all the reagents needed to conduct the assay, including a set of positive control probes for mouse beta Actin (ACTB, NM_007393). Target Probe sets for genes of interest are sold separately.

For the study of human samples, use the Human PrimeFlow RNA Assay (3-plex, cat. no. 88-18009). For the study of alternative species, either kit may be used, however, appropriate positive control probes need to be purchased separately.


Details
Reactivity Mouse
Components PrimeFlow™ RNA Tubes: cat. 19197, store at room temperature
PrimeFlow™ RNA Fixation Buffer 1A: cat. 00-18100, store at 2-8°C
PrimeFlow™ RNA Fixation Buffer 1B: cat. 00-18200, store at 2-8°C
PrimeFlow™ RNA Permeabilization Buffer (10X): cat. 00-18300, store at 2-8°C
PrimeFlow™ RNA Fixation Buffer 2 (8X): cat. 00-18400, store at 2-8°C
PrimeFlow™ RNA Wash Buffer: cat. 00-19180, store at 2-8°C
PrimeFlow™ RNA Target Probe Diluent: cat. 00-19185, store at 2-8°C
PrimeFlow™ RNA PreAmp Mix: cat. 00-19187, store at 2-8°C
PrimeFlow™ RNA Amp Mix: cat. 00-19190, store at 2-8°C
PrimeFlow™ RNA Label Probe Diluent: cat. 00-19183, store at 2-8°C
PrimeFlow™ RNA Storage Buffer: cat. 00-19178, store at 2-8°C
Rnase Inhibitor 2 (100X): cat. 00-18501, store at 2-8°C
PrimeFlow™ RNA Label Probes: cat. 00-19193, store at -20°C
Rnase Inhibitor 1 (1000X): cat. 00-18500, store at -20°C

Positive Control Probe Sets:
Type 1 Mouse ACTB Alexa Fluor® 647 (NM_007393): cat. VB1-10350, store at -20°C
Type 4 Mouse ACTB Alexa Fluor® 488 (NM_007393): cat. VB4-10432, store at -20°C
Type 6 Mouse ACTB Alexa Fluor® 750 (NM_007393): cat. VB6-12823, store at -20°C
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Related Products
References