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Anti-Human/Mouse OCT3/4 Alexa Fluor® 488

Also known as: OCT3, OCT4, octamer-binding transcription factor, POU5F1

Clone: EM92

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 53-5841

Cat. No. Size
53-5841-80 25 ug
53-5841-82 100 ug

Data for Anti-Human/Mouse OCT3/4 Alexa Fluor® 488.

Intracellular staining of F9 cell line with 0.5 ug of Rat IgG2a kappa Isotype Control Alexa Fluor®...View More

  • Data for Anti-Human/Mouse OCT3/4 Alexa Fluor® 488.
Description

Description: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).

Details
Host Rat
Isotype IgG2a, kappa
Reactivity Human, Mouse
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis, Immunocytochemistry
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
41-5841 Anti-Human/Mouse OCT3/4 eFluor® 570 555 nm 570 nm ICC RUO
14-5841 Anti-Human/Mouse OCT3/4 Purified IHC, WB RUO
50-5841 Anti-Human/Mouse OCT3/4 eFluor® 660 633 - 647 nm 668 nm FC RUO
12-5841 Anti-Human/Mouse OCT3/4 PE 488 - 561 nm 578 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
14-8883 Anti-Human TRA-1-81 (Podocalyxin) Purified FC, ICC, IP, WB RUO
00-5521 Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent FC GPR
53-4321 Rat IgG2a K Isotype Control Alexa Fluor® 488 488 nm 519 nm FC, ICC, IHC RUO
References

References: Byrne SM, Ortiz L, Mali P, Aach J, Church GM. Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells. Nucleic Acids Res. 2015 Feb 18;43(3):e21. (EM92, FC, PubMed)

Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990 Feb 9;60(3):461-72.

Pikarsky E, Sharir H, Ben-Shushan E, Bergman Y. Retinoic acid represses Oct-3/4 gene expression through several retinoic acid-responsive elements located in the promoter-enhancer region. Mol Cell Biol. 1994 Feb;14(2):1026-38.

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007 Nov 30;131(5):861-72