Anti-Mouse Nanog Alexa Fluor® 647 (Discontinued. Refer to Cat. No. 50-5761)

Also known as: Homeobox protein NANOG, ES cell-associated protein 4, Early embryo specific expression NK-type homeobox protein, Homeobox transcription factor Nanog

Clone: eBioMLC-51

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: Replacement format is eFluor 660. Select Cat. No. 50-5761.

SKU# 51-5761

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Left: Intracellular staining of fixed and permeabilized F9 cells with 0.03 ug of Rat IgG2a kappa...View More

Description

Description: The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.

Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved.

Immunoblotting using eBioMLC51 reveals a band at ~ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen.

Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse Nanog.

Details

Host	Rat
Isotype	IgG2a
Reactivity	Mouse
Conjugate	Alexa Fluor 647
Laser	Red Laser
Emit	668 nm
Excite	633 - 647 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
53-5761	Anti-Mouse Nanog Alexa Fluor® 488	488 nm	519 nm	FC, ICC	RUO
50-5761	Anti-Mouse Nanog eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC, ICC, IHC, WB	RUO
14-5761	Anti-Mouse Nanog Purified			ICC, WB	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-5521	Foxp3 Fixation/Permeabilization Concentrate and Diluent			FC	GPR
00-5523	Foxp3 / Transcription Factor Staining Buffer Set			FC	GPR
14-5761	Anti-Mouse Nanog Purified			ICC, WB	RUO
51-4321	Rat IgG2a K Isotype Control Alexa Fluor® 647 (To Be Discontinued. Refer to Cat. No. 50-4321)	633 - 647 nm	668 nm	FC, ICC, IHC	RUO
53-5761	Anti-Mouse Nanog Alexa Fluor® 488	488 nm	519 nm	FC, ICC	RUO

References

References: Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., Maruyama, M., Maeda, M., Yamanaka, S. 2003. The Homeoprotein Nanog is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells. Cell 113:631-642

Yanmei Chen, Zhongwei Du, Zhen Yao. 2006. Roles of the Nanog Protein in Murine F9 Embryonal Carcinoma Cells and Their Endoderm-Differentiated Counterparts. Cell Research 16:641-650.

Guangjin Pan and Duanqing Pei. 2005. The Stem Cell Pluripotency Factor NANOG Activates Transcription with Two Unuasually Potent Subdomains at Its C Terminus. The Journal of Biological Chemistry Vol.280, No.2, 1401-1407.

View More Products

Antigens	Area of Biology	Cell Type	Formats
Nanog	Stem Cell Biology Transcription Factors	Embryonic Stem Cells	Alexa Fluor 647