Anti-Mouse MHC Class II (I-A/I-E) PE-Cyanine7

Also known as: MHC II, IA, IE, I-A/E, IA/IE

Clone: M5/114.15.2

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 25-5321

Cat. No.	Size
25-5321-80	25 ug
25-5321-82	100 ug
Please inquire about custom configurations or bulk packaging.

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Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (cat. 17-0031) and 0.06 ug of Rat IgG2b K...View More

Description

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

Details

Host	Rat
Isotype	IgG2b, kappa
Reactivity	Mouse
Conjugate	PE-Cyanine7
Laser	Blue Laser, Green Laser, Yellow-Green Laser
Emit	775 nm
Excite	488 - 561 nm
Legal	FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
15-5321	Anti-Mouse MHC Class II (I-A/I-E) PE-Cyanine5	488 - 561 nm		FC	RUO
48-5321	Anti-Mouse MHC Class II (I-A/I-E) eFluor® 450	405 nm	450 nm	FC	RUO
46-5321	Anti-Mouse MHC Class II (I-A/I-E) PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
36-5321	Anti-Mouse MHC Class II (I-A/I-E) Functional Grade Biotin			FC, IHC, IP	RUO
47-5321	Anti-Mouse MHC Class II (I-A/I-E) APC-eFluor® 780	633 - 647 nm	780 nm	FC	RUO
14-5321	Anti-Mouse MHC Class II (I-A/I-E) Purified			FC, IHC, FA, IP, WB	RUO
11-5321	Anti-Mouse MHC Class II (I-A/I-E) FITC	488 nm	518 nm	FC	RUO
12-5321	Anti-Mouse MHC Class II (I-A/I-E) PE	488 - 561 nm	575 nm	FC	RUO
56-5321	Anti-Mouse MHC Class II (I-A/I-E) Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
95-5321	Anti-Mouse MHC Class II (I-A/I-E) eFluor® 650NC	350 - 635 nm	650 nm	FC	RUO
17-5321	Anti-Mouse MHC Class II (I-A/I-E) APC	633 - 647 nm	660 nm	FC	RUO
16-5321	Anti-Mouse MHC Class II (I-A/I-E) Functional Grade Purified			FC, FA	RUO
13-5321	Anti-Mouse MHC Class II (I-A/I-E) Biotin			FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
17-0031	Anti-Mouse CD3e APC	633 - 647 nm	660 nm	FC	RUO
25-4031	Rat IgG2b K Isotype Control PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO

References

References: Unternaehrer JJ, Chow A, et al. 2007. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 104(1):234-9. (M5/114.15.2, IP, PubMed)

Gueirard, Pascale et al. 2003."Bordetella bronchiseptica Perists in the Nasal Cavities of Mice and Triggers Early Delivery of Dendritic Cells in the Lymph Nodes Draining the Lower and Upper Respiratory Tract". Infection and Immunity 71(7):4137-4143. (M5/114.15.2, IHC paraffin, PubMed)

Bagavant, Sharp et al. 2002. "Induction and Immunohistology of Autoimmune Ovarian Disease in Cynomolgus Macaques (Macaca fascicularis)". Am J Pathol 160:141-149. (M5/114.15.2, IHC, PubMed)

Li C, Siemasko K, et al. 2002. Cooperative interaction of Ig(alpha) and Ig(beta) of the BCR regulates the kinetics and specificity of antigen targeting. Int Immunol. 14(10):1179-91. (M5/114.15.2, IHC frozen and WB, PubMed)

Mediratta SK, Singh N, et al. 1996. Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. Immunology. 89(2):238-44. (M5/114.15.2, FA, PubMed)

Germain, R. N., A. Bhattacharya, et al. 1982. A single monoclonal anti-Ia antibody inhibits antigen-specific T cell proliferation controlled by distinct Ir genes mapping in different H-2 I subregions. J Immunol 128(3): 1409-13.

Bhattacharya, A., M. E. Dorf, et al. 1981. A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol 127(6): 2488-95.

View More Products

Antigens	Area of Biology	Cell Type	Formats
MHC Class II (I-A/I-E)	Adaptive Immunity Innate Immunity	B Cells Dendritic Cells Macrophage & Monocyte Cells T Cells	PE-Cyanine7