Anti-Mouse MHC Class II (I-A/I-E) Biotin

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

References: Unternaehrer JJ, Chow A, et al. 2007. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 104(1):234-9. (M5/114.15.2, IP, PubMed)

Gueirard, Pascale et al. 2003."Bordetella bronchiseptica Perists in the Nasal Cavities of Mice and Triggers Early Delivery of Dendritic Cells in the Lymph Nodes Draining the Lower and Upper Respiratory Tract". Infection and Immunity 71(7):4137-4143. (M5/114.15.2, IHC paraffin, PubMed)

Bagavant, Sharp et al. 2002. "Induction and Immunohistology of Autoimmune Ovarian Disease in Cynomolgus Macaques (Macaca fascicularis)". Am J Pathol 160:141-149. (M5/114.15.2, IHC, PubMed)

Li C, Siemasko K, et al. 2002. Cooperative interaction of Ig(alpha) and Ig(beta) of the BCR regulates the kinetics and specificity of antigen targeting. Int Immunol. 14(10):1179-91. (M5/114.15.2, IHC frozen and WB, PubMed)

Mediratta SK, Singh N, et al. 1996. Analysis of T-cell hybridomas with an unusual MHC class II-dependent ligand specificity. Immunology. 89(2):238-44. (M5/114.15.2, FA, PubMed)

Germain, R. N., A. Bhattacharya, et al. 1982. A single monoclonal anti-Ia antibody inhibits antigen-specific T cell proliferation controlled by distinct Ir genes mapping in different H-2 I subregions. J Immunol 128(3): 1409-13.

Bhattacharya, A., M. E. Dorf, et al. 1981. A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol 127(6): 2488-95.