Anti-Mouse MHC Class Ib (H2-M3) PE

Description: This mAb 130 monoclonal antibody reacts with mouse MHC class Ib H2-M3. This molecule specifically binds N-formylated peptides, which arise from mitochondrial or bacterial proteins. H2-M3-restricted T cells are mainly cytotoxic CD8+ T cells. H2-M3 associates with beta2-microglobulin. Finally, H2-M3 is expressed on cells in the spleen, Peyer’s patches, and lymph nodes, but not in the thymus. More specifically, cell surface expression of H2-M3 is low on B cells. However, H2-M3 can be induced by incubation with N-formylated peptide on T and B cells, macrophages, and dendritic cells.

References: Colmone A, Wang CR. H2-M3-restricted T cell response to infection. Microbes Infect. 2006 Jul;8(8):2277-83.

Chiu NM, Chun T, Fay M, Mandal M, Wang CR. The majority of H2-M3 is retained intracellularly in a peptide-receptive state and traffics to the cell surface in the presence of N-formylated peptides. J Exp Med. 1999 Aug 2;190(3):423-34. (mAb 130, FC, WB,Pubmed)

Lindahl KF, Byers DE, Dabhi VM, Hovik R, Jones EP, Smith GP, Wang CR, Xiao H, Yoshino M. H2-M3, a full-service class Ib histocompatibility antigen. Annu Rev Immunol. 1997;15:851-79.